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Title: Temperature dependent bacteriophages of a tropical bacterial pathogen
Authors: Shan, Jinyu
Korbsrisate, S.
Withatanung, P.
Lazar Adler, Natalie
Clokie, Martha R. J.
Galyov, Edouard E.
First Published: 14-Nov-2014
Citation: Frontiers in Microbiology, 2014, 5:599
Abstract: There is an increasing awareness of the multiple ways that bacteriophages (phages) influence bacterial evolution, population dynamics, physiology, and pathogenicity. By studying a novel group of phages infecting a soil borne pathogen, we revealed a paradigm shifting observation that the phages switch their lifestyle according to temperature. We sampled soil from an endemic area of the serious tropical pathogen Burkholderia pseudomallei, and established that podoviruses infecting the pathogen are frequently present in soil, and many of them are naturally occurring variants of a common virus type. Experiments on one phage in the related model B. thailandensis demonstrated that temperature defines the outcome of phage-bacteria interactions. At higher temperatures (37◦C), the phage predominantly goes through a lytic cycle, but at lower temperatures (25◦C), the phage remains temperate. This is the first report of a naturally occurring phage that follows a lytic or temperate lifestyle according to temperature.These observations fundamentally alter the accepted views on the abundance, population biology and virulence of B. pseudomallei. Furthermore, when taken together with previous studies, our findings suggest that the phenomenon of temperature dependency in phages is widespread. Such phages are likely to have a profound effect on bacterial biology, and on our ability to culture and correctly enumerate viable bacteria.
DOI Link: 10.3389/fmicb.2014.00599
ISSN: 1664-302X
eISSN: 1664-302X
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2014 Shan, Korbsrisate, Withatanung, Adler, Clokie and Galyov. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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