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Title: DNA gyrase can cleave short DNA fragments in the presence of quinolone drugs
Authors: Cove, Matthew E.
Tingey, Andrew P.
Maxwell, Anthony
First Published: 1997
Publisher: Oxford University Press (OUP)
Citation: Nucleic Acids Research, 1997, Vol. 25, No. 14 2716–2722
Abstract: We have analysed the DNA cleavage reaction of DNA gyrase using oligonucleotides annealed to a singlestranded M13 derivative containing a preferred gyrase cleavage site. We find that gyrase can cleave duplexes down to ∼20 bp in size in the presence of the quinolone drugs ciprofloxacin and oxolinic acid. Ciprofloxacin shows a variation in its site specificity with an apparent preference for G bases adjacent to the cleavage sites, whereas oxolinic acid stimulates cleavage predominantly at the previously determined site. With either drug, cleavage will not occur within 6 bases from the end of a DNA duplex or a nick. We suggest that cleavage site specificity with short DNA duplexes is determined by drug-DNA interactions whereas with longer fragments the positioning effect of the DNA wrap around gyrase prescribes the site of cleavage.
DOI Link: 10.1093/nar/25.14.2716
ISSN: 0305-1048
eISSN: 1362-4962
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Archived with reference to SHERPA/RoMEO and publisher website. © 1997 Oxford University Press. Version of record:
Appears in Collections:Published Articles, Dept. of Biochemistry

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