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|Title:||DNA gyrase can cleave short DNA fragments in the presence of quinolone drugs|
|Authors:||Cove, Matthew E.|
Tingey, Andrew P.
|Publisher:||Oxford University Press (OUP)|
|Citation:||Nucleic Acids Research, 1997, Vol. 25, No. 14 2716–2722|
|Abstract:||We have analysed the DNA cleavage reaction of DNA gyrase using oligonucleotides annealed to a singlestranded M13 derivative containing a preferred gyrase cleavage site. We find that gyrase can cleave duplexes down to ∼20 bp in size in the presence of the quinolone drugs ciprofloxacin and oxolinic acid. Ciprofloxacin shows a variation in its site specificity with an apparent preference for G bases adjacent to the cleavage sites, whereas oxolinic acid stimulates cleavage predominantly at the previously determined site. With either drug, cleavage will not occur within 6 bases from the end of a DNA duplex or a nick. We suggest that cleavage site specificity with short DNA duplexes is determined by drug-DNA interactions whereas with longer fragments the positioning effect of the DNA wrap around gyrase prescribes the site of cleavage.|
|Rights:||Archived with reference to SHERPA/RoMEO and publisher website. © 1997 Oxford University Press. Version of record: http://nar.oxfordjournals.org/content/25/14/2716|
|Appears in Collections:||Published Articles, Dept. of Biochemistry|
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