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|Title:||Mechanical activation of vinculin binding to talin locks talin in an unfolded conformation.|
Goult, Benjamin T.
Sheetz, M. P.
|Publisher:||Nature Publishing Group|
|Citation:||Scientific Reports, 2014, 4:4610|
|Abstract:||The force-dependent interaction between talin and vinculin plays a crucial role in the initiation and growth of focal adhesions. Here we use magnetic tweezers to characterise the mechano-sensitive compact N-terminal region of the talin rod, and show that the three helical bundles R1-R3 in this region unfold in three distinct steps consistent with the domains unfolding independently. Mechanical stretching of talin R1-R3 enhances its binding to vinculin and vinculin binding inhibits talin refolding after force is released. Mutations that stabilize R3 identify it as the initial mechano-sensing domain in talin, unfolding at ∼5 pN, suggesting that 5 pN is the force threshold for vinculin binding and adhesion progression.|
|Rights:||This work is licensed under a Creative Commons Attribution-NonCommercialShareAlike 3.0 Unported License (CC BY-NC-SA 3.0). The images in this article are included in the article’s Creative Commons license, unless indicated otherwise in the image credit; if the image is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the image. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/|
|Appears in Collections:||Published Articles, Dept. of Biochemistry|
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