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Title: Immune stimulation reduces sleep and memory ability in Drosophila melanogaster.
Authors: Mallon, Eamonn B.
Alghamdi, A.
Holdbrook, Robert T.K.
Rosato, Ezio
First Published: 10-Jun-2014
Publisher: PeerJ
Citation: PeerJ, 2014, 2, pp. 2:e434;
Abstract: Psychoneuroimmunology studies the increasing number of connections between neurobiology, immunology and behaviour. We demonstrate the effects of the immune response on two fundamental behaviours: sleep and memory ability in Drosophila melanogaster. We used the Geneswitch system to upregulate peptidoglycan receptor protein (PGRP) expression, thereby stimulating the immune system in the absence of infection. Geneswitch was activated by feeding the steroid RU486, to the flies. We used an aversive classical conditioning paradigm to quantify memory and measures of activity to infer sleep. Immune stimulated flies exhibited reduced levels of sleep, which could not be explained by a generalised increase in waking activity. Immune stimulated flies also showed a reduction in memory abilities. These results lend support to Drosophila as a model for immune-neural interactions and provide a possible role for sleep in the interplay between the immune response and memory.
DOI Link: 10.7717/peerj.434
ISSN: 2167-8359
eISSN: 2167-8359
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: © 2014 Mallon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
Description: PMCID: PMC4060034 Complete ANOVAs DOI: 10.7717/peerj.434/supp-1
Appears in Collections:Published Articles, Dept. of Biology

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