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Title: Host Glycan Sugar-Specific Pathways in Streptococcus pneumoniae: Galactose as a Key Sugar in Colonisation and Infection.
Authors: Paixão, L.
Oliveira, J.
Veríssimo, A.
Vinga, S.
Lourenço, E. C.
Ventura, M. R.
Kjos, M.
Veening, J. W.
Fernandes, Vitor E.
Andrew, Peter W.
Yesilkaya, Hasan
Neves, A. R.
First Published: 31-Mar-2015
Publisher: Public Library of Science
Citation: PLoS One, 2015, 10 (3), e0121042
Abstract: The human pathogen Streptococcus pneumoniae is a strictly fermentative organism that relies on glycolytic metabolism to obtain energy. In the human nasopharynx S. pneumoniae encounters glycoconjugates composed of a variety of monosaccharides, which can potentially be used as nutrients once depolymerized by glycosidases. Therefore, it is reasonable to hypothesise that the pneumococcus would rely on these glycan-derived sugars to grow. Here, we identified the sugar-specific catabolic pathways used by S. pneumoniae during growth on mucin. Transcriptome analysis of cells grown on mucin showed specific upregulation of genes likely to be involved in deglycosylation, transport and catabolism of galactose, mannose and N acetylglucosamine. In contrast to growth on mannose and N-acetylglucosamine, S. pneumoniae grown on galactose re-route their metabolic pathway from homolactic fermentation to a truly mixed acid fermentation regime. By measuring intracellular metabolites, enzymatic activities and mutant analysis, we provide an accurate map of the biochemical pathways for galactose, mannose and N-acetylglucosamine catabolism in S. pneumoniae. Intranasal mouse infection models of pneumococcal colonisation and disease showed that only mutants in galactose catabolic genes were attenuated. Our data pinpoint galactose as a key nutrient for growth in the respiratory tract and highlights the importance of central carbon metabolism for pneumococcal pathogenesis.
DOI Link: 10.1371/journal.pone.0121042
ISSN: 1932-6203
eISSN: 1932-6203
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright: © 2015 Paixão et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Description: PMCID: PMC4380338 Corrected title as per Data Availability Statement: Except for microarray data, all other relevant data are within the paper and its Supporting Information files. Fully annotated microarray data have been deposited in BµG@Sbase (accession number E-BUGS-159; uk/E-BUGS-159) and also ArrayExpress (accession number E-BUGS-159).
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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