Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/32248
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dc.contributor.authorPaixão, L.-
dc.contributor.authorOliveira, J.-
dc.contributor.authorVeríssimo, A.-
dc.contributor.authorVinga, S.-
dc.contributor.authorLourenço, E. C.-
dc.contributor.authorVentura, M. R.-
dc.contributor.authorKjos, M.-
dc.contributor.authorVeening, J. W.-
dc.contributor.authorFernandes, Vitor E.-
dc.contributor.authorAndrew, Peter W.-
dc.contributor.authorYesilkaya, Hasan-
dc.contributor.authorNeves, A. R.-
dc.date.accessioned2015-05-08T13:38:01Z-
dc.date.available2015-05-08T13:38:01Z-
dc.date.issued2015-03-31-
dc.identifier.citationPLoS One, 2015, 10 (3), e0121042en
dc.identifier.issn1932-6203-
dc.identifier.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0121042en
dc.identifier.urihttp://hdl.handle.net/2381/32248-
dc.descriptionPMCID: PMC4380338 Corrected title as per http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0127483 Data Availability Statement: Except for microarray data, all other relevant data are within the paper and its Supporting Information files. Fully annotated microarray data have been deposited in BµG@Sbase (accession number E-BUGS-159; http://bugs.sgul.ac. uk/E-BUGS-159) and also ArrayExpress (accession number E-BUGS-159).en
dc.description.abstractThe human pathogen Streptococcus pneumoniae is a strictly fermentative organism that relies on glycolytic metabolism to obtain energy. In the human nasopharynx S. pneumoniae encounters glycoconjugates composed of a variety of monosaccharides, which can potentially be used as nutrients once depolymerized by glycosidases. Therefore, it is reasonable to hypothesise that the pneumococcus would rely on these glycan-derived sugars to grow. Here, we identified the sugar-specific catabolic pathways used by S. pneumoniae during growth on mucin. Transcriptome analysis of cells grown on mucin showed specific upregulation of genes likely to be involved in deglycosylation, transport and catabolism of galactose, mannose and N acetylglucosamine. In contrast to growth on mannose and N-acetylglucosamine, S. pneumoniae grown on galactose re-route their metabolic pathway from homolactic fermentation to a truly mixed acid fermentation regime. By measuring intracellular metabolites, enzymatic activities and mutant analysis, we provide an accurate map of the biochemical pathways for galactose, mannose and N-acetylglucosamine catabolism in S. pneumoniae. Intranasal mouse infection models of pneumococcal colonisation and disease showed that only mutants in galactose catabolic genes were attenuated. Our data pinpoint galactose as a key nutrient for growth in the respiratory tract and highlights the importance of central carbon metabolism for pneumococcal pathogenesis.en
dc.description.sponsorshipThis work was supported by Fundação para a Ciência e a Tecnologia, Portugal (FCT) and FEDER, project PTDC/SAU-MII/100964/2008, and through grants PEst-OE/EQB/LA0004/2011 and IDMEC, under LAETA Pest-OE/EME/LA0022. L. Paixão, E. C. Lourenço and A. Veríssimo acknowledge FCT for the award of Ph.D. grants SFRH/BD/46997/2008, SFRH/47702/2008 and SFRH/BD/97415/2013, respectively. M. Kjos was supported by a FEBS Long-term fellowship. Work in the lab of J.-W. Veening was supported by European Research Council Starting grant (337399-PneumoCell) and a Vidi fellowship (864.12.001) from the Netherlands Organisation for Scientific Research (NWO-ALW). S. Vinga acknowledges support by Program Investigator FCT (IF/00653/2012) from FCT, co-funded by the European Social Fund (ESF) through the Operational Program Human Potential (POPH). The NMR spectrometers are part of The National NMR Facility, supported by Fundação para a Ciência e a Tecnologia (RECI/BBB-BQB/0230/2012).en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.relation.urihttp://www.ncbi.nlm.nih.gov/pubmed/25826206-
dc.rightsCopyright: © 2015 Paixão et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.titleHost Glycan Sugar-Specific Pathways in Streptococcus pneumoniae: Galactose as a Key Sugar in Colonisation and Infection.en
dc.typeJournal Articleen
dc.identifier.doi10.1371/journal.pone.0121042-
dc.identifier.eissn1932-6203-
dc.identifier.piiPONE-D-14-57261-
dc.description.statusPeer-revieweden
dc.description.versionPublisher Versionen
dc.type.subtypeJournal Article-
pubs.organisational-group/Organisationen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGYen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicineen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine/Department of Infection, Immunity and Inflammationen
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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