Please use this identifier to cite or link to this item:
|Title:||Hsp72 is targeted to the mitotic spindle by Nek6 to promote K-fiber assembly and mitotic progression.|
Richards, Mark W.
Hood, F. E.
Royle, S. J.
Fry, Andrew M.
|Publisher:||Rockefeller University Press|
|Citation:||Journal of Cell Biology 2015 vol. 209 no. 3 349-358|
|Abstract:||Hsp70 proteins represent a family of chaperones that regulate cellular homeostasis and are required for cancer cell survival. However, their function and regulation in mitosis remain unknown. In this paper, we show that the major inducible cytoplasmic Hsp70 isoform, Hsp72, is required for assembly of a robust bipolar spindle capable of efficient chromosome congression. Mechanistically, Hsp72 associates with the K-fiber-stabilizing proteins, ch-TOG and TACC3, and promotes their interaction with each other and recruitment to spindle microtubules (MTs). Targeting of Hsp72 to the mitotic spindle is dependent on phosphorylation at Thr-66 within its nucleotide-binding domain by the Nek6 kinase. Phosphorylated Hsp72 concentrates on spindle poles and sites of MT-kinetochore attachment. A phosphomimetic Hsp72 mutant rescued defects in K-fiber assembly, ch-TOG/TACC3 recruitment and mitotic progression that also resulted from Nek6 depletion. We therefore propose that Nek6 facilitates association of Hsp72 with the mitotic spindle, where it promotes stable K-fiber assembly through recruitment of the ch-TOG-TACC3 complex.|
|Rights:||Archived with reference to SHERPA/RoMEO and publisher website. © 2015 O’Regan et al. This article is distributed under the terms of an Attribution– Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).|
|Description:||Supplemental material can be found at: http://jcb.rupress.org/content/suppl/2015/04/30/jcb.201409151.DC1.html|
|Appears in Collections:||Published Articles, Dept. of Biochemistry|
Files in This Item:
|hsp72.pdf||Published (publisher PDF)||4.88 MB||Adobe PDF||View/Open|
Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.