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Title: Molecular mechanisms of human IRE1 activation through dimerization and ligand binding
Authors: Joshi, Amar
Newbatt, Y
McAndrew, P. C.
Stubbs, M.
Burke, R.
Richards, Mark W.
Bhatia, Chitra
Caldwell, J. J.
McHardy, T.
Collins, I.
Bayliss, Richard
First Published: 18-Apr-2015
Publisher: Impact Journals
Citation: Oncotarget, 2015
Abstract: IRE1 transduces the unfolded protein response by splicing XBP1 through its C-terminal cytoplasmic kinase-RNase region. IRE1 autophosphorylation is coupled to RNase activity through formation of a back-to-back dimer, although the conservation of the underlying molecular mechanism is not clear from existing structures. We have crystallized human IRE1 in a back-to-back conformation only previously seen for the yeast homologue. In our structure the kinase domain appears primed for catalysis but the RNase domains are disengaged. Structure-function analysis reveals that IRE1 is autoinhibited through a Tyr-down mechanism related to that found in the unrelated Ser/Thr protein kinase Nek7. We have developed a compound that potently inhibits human IRE1 kinase activity while stimulating XBP1 splicing. A crystal structure of the inhibitor bound to IRE1 shows an increased ordering of the kinase activation loop. The structures of hIRE in apo and ligand-bound forms are consistent with a previously proposed model of IRE1 regulation in which formation of a back-to-back dimer coupled to adoption of a kinase-active conformation drive RNase activation. The structures provide opportunities for structure-guided design of IRE1 inhibitors
DOI Link: 10.18632/oncotarget.3864
eISSN: 1949-2553
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Creative Commons Attribution 3.0 License. (CC BY 3.0) (
Description: PMID: 25968568 [PubMed - as supplied by publisher]. Atomic coordinates and structure factors for apohIRE1 and compound 3-hIRE have been deposited in the PDB, accession codes 4Z7G and 4Z7H respectively.
Appears in Collections:Published Articles, Dept. of Biochemistry

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