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|Title:||The AgI/II Family Adhesin AspA Is Required for Respiratory Infection by Streptococcus pyogenes|
Nobbs, A. H.
Wright, C. J.
Maddocks, S. E.
Sahota, J. S.
Jenkinson, H. F.
|Publisher:||Public Library of Science|
|Citation:||PLoS One, 2013, 8 (4), e62433|
|Abstract:||Streptococcus pyogenes (GAS) is a human pathogen that causes pharyngitis and invasive diseases such as toxic shock syndrome and sepsis. The upper respiratory tract is the primary reservoir from which GAS can infect new hosts and cause disease. The factors involved in colonisation are incompletely known however. Previous evidence in oral streptococci has shown that the AgI/II family proteins are involved. We hypothesized that the AspA member of this family might be involved in GAS colonization. We describe a novel mouse model of GAS colonization of the nasopharynx and lower respiratory tract to elucidate these interactions. We used two clinical M serotypes expressing AspA, and their aspA gene deletant isogenic mutants in experiments using adherence assays to respiratory epithelium, macrophage phagocytosis and neutrophil killing assays and in vivo models of respiratory tract colonisation and infection. We demonstrated the requirement for AspA in colonization of the respiratory tract. AspA mutants were cleared from the respiratory tract and were deficient in adherence to epithelial cells, and susceptible to phagocytosis. Expression of AspA in the surrogate host Lactococcus lactis protected bacteria from phagocytosis. Our results suggest that AspA has an essential role in respiratory infection, and may function as a novel anti-phagocytic factor.|
|Rights:||Copyright © 2013 Franklin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
|Description:||Correction: 17 Sep 2013: Franklin L, Nobbs AH, Bricio-Moreno L, Wright CJ, Maddocks SE, et al. (2013) Correction: The AgI/II Family Adhesin AspA Is Required for Respiratory Infection by Streptococcus pyogenes. PLoS ONE 8(9): 10.1371/annotation/8b573590-d849-46b0-8cdf-277d125b4478 Available at http://journals.plos.org/plosone/article?id=10.1371/annotation/8b573590-d849-46b0-8cdf-277d125b4478|
|Appears in Collections:||Published Articles, Dept. of Infection, Immunity and Inflammation|
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