Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/32709
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dc.contributor.authorLucidarme, J.-
dc.contributor.authorFindlow, J.-
dc.contributor.authorChan, H.-
dc.contributor.authorFeavers, I. M.-
dc.contributor.authorGray, S. J.-
dc.contributor.authorKaczmarski, E. B.-
dc.contributor.authorParkhill, J.-
dc.contributor.authorBai, X.-
dc.contributor.authorBorrow, R.-
dc.contributor.authorBayliss, Christopher D.-
dc.date.accessioned2015-07-20T08:37:46Z-
dc.date.available2015-07-20T08:37:46Z-
dc.date.issued2013-09-30-
dc.identifier.citationPLoS One, 2013, 8 (9), e76932en
dc.identifier.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0076932en
dc.identifier.urihttp://hdl.handle.net/2381/32709-
dc.description.abstractTwo haemoglobin-binding proteins, HmbR and HpuAB, contribute to iron acquisition by Neisseria meningitidis. These receptors are subject to high frequency, reversible switches in gene expression--phase variation (PV)--due to mutations in homopolymeric (poly-G) repeats present in the open reading frame. The distribution and PV state of these receptors was assessed for a representative collection of isolates from invasive meningococcal disease patients of England, Wales and Northern Ireland. Most of the major clonal complexes had only the HmbR receptor whilst the recently expanding ST-275-centred cluster of the ST-269 clonal complex had both receptors. At least one of the receptors was in an 'ON' configuration in 76.3% of the isolates, a finding that was largely consistent with phenotypic analyses. As PV status may change during isolation and culture of meningococci, a PCR-based protocol was utilised to confirm the expression status of the receptors within contemporaneously acquired clinical specimens (blood/cerebrospinal fluid) from the respective patients. The expression state was confirmed for all isolate/specimen pairs with <15 tract repeats indicating that the PV status of these receptors is stable during isolation. This study therefore establishes a protocol for determining in vivo PV status to aid in determining the contributions of phase variable genes to invasive meningococcal disease. Furthermore, the results of the study support a putative but non-essential role of the meningococcal haemoglobin receptors as virulence factors whilst further highlighting their vaccine candidacy.en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.relation.urihttp://www.ncbi.nlm.nih.gov/pubmed/24098814-
dc.rightsCopyright © 2013 Lucidarme et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectBacterial Outer Membrane Proteinsen
dc.subjectBacterial Proteinsen
dc.subjectGene Expression Regulation, Bacterialen
dc.subjectGenotypeen
dc.subjectGreat Britainen
dc.subjectHemoglobinsen
dc.subjectHumansen
dc.subjectIronen
dc.subjectMeningitis, Meningococcalen
dc.subjectPhenotypeen
dc.subjectReceptors, Cell Surfaceen
dc.subjectSequence Analysis, DNAen
dc.subjectVirulence Factorsen
dc.titleThe Distribution and ‘In Vivo’ Phase Variation Status of Haemoglobin Receptors in Invasive Meningococcal Serogroup B Disease: Genotypic and Phenotypic Analysisen
dc.typeJournal Articleen
dc.identifier.doi10.1371/journal.pone.0076932-
dc.identifier.eissn1932-6203-
dc.identifier.piiPONE-D-13-19665-
dc.description.statusPeer-revieweden
dc.description.versionPublisher Versionen
dc.type.subtypeJournal Article;Research Support, Non-U.S. Gov't-
pubs.organisational-group/Organisationen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGYen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Biological Sciencesen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Biological Sciences/Department of Geneticsen
dc.dateaccepted2013-09-05-
Appears in Collections:Published Articles, Dept. of Genetics



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