Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/32712
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dc.contributor.authorLazar Adler, Natalie R.-
dc.contributor.authorDean, R. E.-
dc.contributor.authorSaint, R. J.-
dc.contributor.authorStevens, M. P.-
dc.contributor.authorPrior, J. L.-
dc.contributor.authorAtkins, T. P.-
dc.contributor.authorGalyov, Edouard E.-
dc.date.accessioned2015-07-20T08:55:01Z-
dc.date.available2015-07-20T08:55:01Z-
dc.date.issued2013-11-05-
dc.identifier.citationPLoS One, 2013, 8 (11), e79461en
dc.identifier.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0079461en
dc.identifier.urihttp://hdl.handle.net/2381/32712-
dc.description.abstractThe autotransporters are a large and diverse family of bacterial secreted and outer membrane proteins, which are present in many Gram-negative bacterial pathogens and play a role in numerous environmental and virulence-associated interactions. As part of a larger systematic study on the autotransporters of Burkholderia pseudomallei, the causative agent of the severe tropical disease melioidosis, we have constructed an insertion mutant in the bpss1439 gene encoding an unstudied predicted trimeric autotransporter adhesin. The bpss1439 mutant demonstrated a significant reduction in biofilm formation at 48 hours in comparison to its parent 10276 wild-type strain. This phenotype was complemented to wild-type levels by the introduction of a full-length copy of the bpss1439 gene in trans. Examination of the wild-type and bpss1439 mutant strains under biofilm-inducing conditions by microscopy after 48 hours confirmed that the bpss1439 mutant produced less biofilm compared to wild-type. Additionally, it was observed that this phenotype was due to low levels of bacterial adhesion to the abiotic surface as well as reduced microcolony formation. In a murine melioidosis model, the bpss1439 mutant strain demonstrated a moderate attenuation for virulence compared to the wild-type strain. This attenuation was abrogated by in trans complementation, suggesting that bpss1439 plays a subtle role in the pathogenesis of B. pseudomallei. Taken together, these studies indicate that BPSS1439 is a novel predicted autotransporter involved in biofilm formation of B. pseudomallei; hence, this factor was named BbfA, Burkholderia biofilm factor A.en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.relation.urihttp://www.ncbi.nlm.nih.gov/pubmed/24223950-
dc.rightsCopyright © 2013 Lazar Adler et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectAdhesins, Bacterialen
dc.subjectAnimalsen
dc.subjectBacterial Adhesionen
dc.subjectBiofilmsen
dc.subjectBurkholderia pseudomalleien
dc.subjectMelioidosisen
dc.subjectMiceen
dc.subjectMutationen
dc.subjectPhenotypeen
dc.subjectProtein Multimerizationen
dc.subjectProtein Structure, Quaternaryen
dc.titleIdentification of a predicted trimeric autotransporter adhesin required for biofilm formation of Burkholderia pseudomallei.en
dc.typeJournal Articleen
dc.identifier.doi10.1371/journal.pone.0079461-
dc.identifier.eissn1932-6203-
dc.identifier.piiPONE-D-13-18563-
dc.description.statusPeer-revieweden
dc.description.versionPublisher Versionen
dc.type.subtypeJournal Article;Research Support, Non-U.S. Gov't-
pubs.organisational-group/Organisationen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGYen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicineen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine/Department of Infection, Immunity and Inflammationen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/Themesen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/Themes/Microbial Scienceen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/Themes/Molecular & Cellular Bioscienceen
dc.dateaccepted2013-09-23-
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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