Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/32842
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dc.contributor.authorDuffy, S. Mark-
dc.contributor.authorAshmole, Ian-
dc.contributor.authorSmallwood, Dawn T.-
dc.contributor.authorLeyland, Mark L.-
dc.contributor.authorBradding, Peter-
dc.date.accessioned2015-07-29T14:06:20Z-
dc.date.available2015-07-29T14:06:20Z-
dc.date.issued2015-07-16-
dc.identifier.citationCell Communication and Signaling, 2015, 13 (1), 32en
dc.identifier.urihttp://www.biosignaling.com/content/13/1/32en
dc.identifier.urihttp://hdl.handle.net/2381/32842-
dc.description.abstractBACKGROUND: Orai/CRACM1 ion channels provide the major Ca[superscript: 2+] influx pathway for FcεRI-dependent human lung mast cell (HLMC) mediator release. The Ca[superscript: 2+]-activated K[superscript: +] channel K[subscript: Ca]3.1 modulates Ca[superscript: 2+] influx and the secretory response through hyperpolarisation of the plasma membrane. We hypothesised that there is a close functional and spatiotemporal interaction between these Ca[superscript: 2+]- and K[superscript: +]-selective channels. RESULTS: Activation of FcεRI-dependent HLMC K[subscript: Ca]3.1 currents was dependent on the presence of extracellular Ca[superscript: 2+], and attenuated in the presence of the selective Orai blocker GSK-7975A. Currents elicited by the K[subscript: Ca]3.1 opener 1-EBIO were also attenuated by GSK-7975A. The Orai1 E106Q dominant-negative mutant ablated 1-EBIO and FcεRI-dependent K[subscript: Ca]3.1 currents in HLMCs. Orai1 but not Orai2 was shown to co-immunoprecipitate with K[subscript: Ca]3.1 when overexpressed in HEK293 cells, and Orai1 and K[subscript: Ca]3.1 were seen to co-localise in the HEK293 plasma membrane using confocal microscopy. CONCLUSION: K[subscript: Ca]3.1 activation in HLMCs is highly dependent on Ca[superscript: 2+] influx through Orai1 channels, mediated via a close spatiotemporal interaction between the two channels.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urihttp://www.ncbi.nlm.nih.gov/pubmed/26177720-
dc.rightsCopyright © 2015 Duffy et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en
dc.subjectOraien
dc.subjectKCa3.1en
dc.subjectMast cellen
dc.titleOrai/CRACM1 and KCa3.1 ion channels interact in the human lung mast cell plasma membraneen
dc.typeJournal Articleen
dc.identifier.doi10.1186/s12964-015-0112-z-
dc.identifier.eissn1478-811X-
dc.identifier.pii10.1186/s12964-015-0112-z-
dc.description.statusPeer-revieweden
dc.description.versionPublisher Versionen
dc.type.subtypeJournal Article-
pubs.organisational-group/Organisationen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGYen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicineen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine/Department of Infection, Immunity and Inflammationen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/Themesen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/Themes/Molecular & Cellular Bioscienceen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/Themes/Respiratory Scienceen
dc.dateaccepted2015-07-09-
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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