Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/32843
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dc.contributor.authorWillets, Jonathon Mark-
dc.contributor.authorBrighton, Paul J.-
dc.contributor.authorWindell, LN-
dc.contributor.authorRana, S-
dc.contributor.authorNash, Craig A.-
dc.contributor.authorKonje, Justin Chi-
dc.date.accessioned2015-07-29T14:52:14Z-
dc.date.available2016-03-09T02:45:08Z-
dc.date.issued2015-05-15-
dc.identifier.citationMolecular and Cellular Endocrinology, 2015, 407, pp. 57-66en
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S030372071500115Xen
dc.identifier.urihttp://hdl.handle.net/2381/32843-
dc.description.abstractBradykinin is associated with infections and inflammation, which given the strong correlation between uterine infection and preterm labour may imply that it could play a role in this process. Therefore, we investigated bradykinin signalling, and the roles that arrestin proteins play in their regulation in human myometrial cells. Bradykinin induced rapid, transient intracellular Ca[superscript: 2+] increases that were inhibited following B[subscript: 2] receptor (B[subscript: 2]R) antagonism. Arrestin2 or arrestin3 depletion enhanced and prolonged bradykinin-stimulated Ca[superscript: 2+] responses, and attenuated B[subscript: 2]R desensitisation. Knockdown of either arrestin enhanced B[subscript: 2]R-stimulated ERK1/2 signals. Moreover, depletion of either arrestin elevated peak-phase p38-MAPK signalling, yet only arrestin3 depletion prolonged B[subscript: 2]R-induced p38-MAPK signals. Arrestin2-knockdown augmented bradykinin-induced cell movement. Bradykinin stimulates pro-contractile signalling mechanisms in human myometrial cells and arrestin proteins play key roles in their regulation. Our data suggest bradykinin not only acts as an utertonin, but may also have the potential to enhance the contractile environment of the uterus.en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urihttp://www.ncbi.nlm.nih.gov/pubmed/25766502-
dc.rightsCopyright © 2015 Elsevier Ireland Ltd. All rights reserved.en
dc.subjectArrestinen
dc.subjectB(2) receptoren
dc.subjectBradykininen
dc.subjectMigrationen
dc.subjectMyometrial contractionen
dc.subjectMyometriumen
dc.titleBradykinin-activated contractile signalling pathways in human myometrial cells are differentially regulated by arrestin proteins.en
dc.typeJournal Articleen
dc.identifier.doi10.1016/j.mce.2015.03.004-
dc.identifier.eissn1872-8057-
dc.identifier.piiS0303-7207(15)00115-X-
dc.description.statusPeer-revieweden
dc.description.versionPost-review (final submitted)en
dc.type.subtypeJournal Article-
pubs.organisational-group/Organisationen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGYen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicineen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine/Department of Cancer Studies and Molecular Medicineen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/Themesen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/Themes/Molecular & Cellular Bioscienceen
dc.dateaccepted2015-03-04-
Appears in Collections:Published Articles, Dept. of Cancer Studies and Molecular Medicine

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