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Title: The external PASTA domain of the essential serine/threonine protein kinase PknB regulates mycobacterial growth
Authors: Turapov, Obolbek
Loraine, Jessica
Jenkins, Christopher H.
Barthe, P.
McFeely, Daniel
Forti, F.
Ghisotti, D.
Hesek, D.
Lee, M.
Bottrill, Andrew R.
Vollmer, W.
Mobashery, S.
Cohen-Gonsaud, M.
Mukamolova, Galina V.
First Published: 1-Jul-2015
Publisher: Royal Society, The: Open Access
Citation: Open Biology, 2015, 5 (7)
Abstract: PknB is an essential serine/threonine protein kinase required for mycobacterial cell division and cell-wall biosynthesis. Here we demonstrate that overexpression of the external PknB_PASTA domain in mycobacteria results in delayed regrowth, accumulation of elongated bacteria and increased sensitivity to β-lactam antibiotics. These changes are accompanied by altered production of certain enzymes involved in cell-wall biosynthesis as revealed by proteomics studies. The growth inhibition caused by overexpression of the PknB_PASTA domain is completely abolished by enhanced concentration of magnesium ions, but not muropeptides. Finally, we show that the addition of recombinant PASTA domain could prevent regrowth of Mycobacterium tuberculosis, and therefore offers an alternative opportunity to control replication of this pathogen. These results suggest that the PknB_PASTA domain is involved in regulation of peptidoglycan biosynthesis and maintenance of cell-wall architecture.
DOI Link: 10.1098/rsob.150025
eISSN: 2046-2441
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2015 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License, which permits unrestricted use, provided the original author and source are credited.
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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