Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/33197
Title: Septicaemia models using Streptococcus pneumoniae and Listeria monocytogenes: understanding the role of complement properdin.
Authors: Dupont, A.
Mohamed, F.
Salehen, N.
Glenn, S.
Francescut, L.
Adib, R.
Byrne, S.
Brewin, H.
Elliott, I.
Richards, L.
Dimitrova, P.
Schwaeble, W.
Ivanovska, N.
Kadioglu, A.
Machado, L. R.
Andrew, Peter W.
Stover, C.
First Published: 12-Apr-2014
Publisher: Springer Berlin Heidelberg
Citation: Medical Microbiology and Immunology, 2014, 203 (4), pp. 257-271
Abstract: Streptococcus pneumoniae and Listeria monocytogenes, pathogens which can cause severe infectious disease in human, were used to infect properdin-deficient and wildtype mice. The aim was to deduce a role for properdin, positive regulator of the alternative pathway of complement activation, by comparing and contrasting the immune response of the two genotypes in vivo. We show that properdin-deficient and wildtype mice mounted antipneumococcal serotype-specific IgM antibodies, which were protective. Properdin-deficient mice, however, had increased survival in the model of streptococcal pneumonia and sepsis. Low activity of the classical pathway of complement and modulation of FcγR2b expression appear to be pathogenically involved. In listeriosis, however, properdin-deficient mice had reduced survival and a dendritic cell population that was impaired in maturation and activity. In vitro analyses of splenocytes and bone marrow-derived myeloid cells support the view that the opposing outcomes of properdin-deficient and wildtype mice in these two infection models is likely to be due to a skewing of macrophage activity to an M2 phenotype in the properdin-deficient mice. The phenotypes observed thus appear to reflect the extent to which M2- or M1-polarised macrophages are involved in the immune responses to S. pneumoniae and L. monocytogenes. We conclude that properdin controls the strength of immune responses by affecting humoral as well as cellular phenotypes during acute bacterial infection and ensuing inflammation.
DOI Link: 10.1007/s00430-013-0324-z
eISSN: 1432-1831
Links: http://link.springer.com/article/10.1007%2Fs00430-013-0324-z
http://hdl.handle.net/2381/33197
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright ©the author(s) 2014. this article is published with open access at springerlink.com. Deposited in accordance with the Publisher's self-archiving policy available at http://www.springer.com/gp/open-access/authors-rights/self-archiving-policy/2124
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

Files in This Item:
File Description SizeFormat 
Septicaemia models using Streptococcus pneumoniae and Listeria monocytogenes: understanding the role of complement properdin..pdfPublished (publisher PDF)2.93 MBAdobe PDFView/Open


Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.