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Title: The relationship between biomarkers of oxidative DNA damage, polycyclic aromatic hydrocarbon DNA adducts, antioxidant status and genetic susceptibility following exposure to environmental air pollution in humans
Authors: Singh, Rajinder
Sram, Radim J.
Binkova, Blanka
Kalina, Ivan
Popov, Todor A.
Georgieva, Tzveta
Garte, Seymour
Taioli, Emanuela
Farmer, Peter B.
First Published: 3-Mar-2007
Publisher: Elsevier
Citation: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 2007, 620(1-2), pp.83-92
Abstract: Polycyclic aromatic hydrocarbons (PAHs) appear to be significant contributors to the genotoxicity and carcinogenicity of air pollution present in the urban environment for humans. Populations exposed to environmental air pollution show increased levels of PAH DNA adducts and it has been postulated that another contributing cause of carcinogenicity by environmental air pollution may be the production of reactive oxygen species following oxidative stress leading to oxidative DNA damage. The antioxidant status as well as the genetic profile of an individual should in theory govern the amount of protection afforded against the deleterious effects associated with exposure to environmental air pollution. In this study we investigated the formation of total PAH (bulky) and B[a]P DNA adducts following exposure of individuals to environmental air pollution in three metropolitan cities and the effect on endogenously derived oxidative DNA damage. Furthermore the influence of antioxidant status (vitamin levels) and genetic susceptibility of individuals with regard to DNA damage was also investigated. There was no significant correlation for individuals between the levels of vitamin A, vitamin E, vitamin C and folate with M1dG and 8-oxodG adducts as well as M1dG adducts with total PAH (bulky) or B[a]P DNA adducts. The interesting find from this study was the significant negative correlation between the level of 8-oxodG adducts and the level of total PAH (bulky) and B[a]P DNA adducts implying the that the repair of oxidative DNA damage may be enhanced. This correlation was most significant for those individuals that were non smokers or those unexposed to environmental air pollution. Furthermore the significant inverse correlation between 8-oxodG and B[a]P DNA adducts was confined to individuals carrying the wild type genotype for both the GSTM1 and the GSTT1 gene (separately and interacting). This effect was not observed for individuals carrying the null variant.
Type: Article
Description: This is the authors' final draft of the paper published as Mutation Research 2007, 620(1-2), pp.83-92 and includes the revised Scheme 1 which appeared as a corrigendum in Mutation Research 2007, 625(1-2), pp.177-78. The final published version of the original paper is available on Science Direct, doi:10.1016/j.mrfmmm.2007.02.025.
Appears in Collections:Published Articles, MRC Toxicology Unit

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