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Title: The role of hyaluronidase in the concentrating kidney.
Authors: Rowen, David.
Award date: 1979
Presented at: University of Leicester
Abstract: It has been postulated that the enzyme hyaluronidase facilitates water reabsorption in the renal collecting duct by reducing the intercellular mucopolysaccharide level and enhancing epithelial permeability. The present study was designed to examine the eflects of enzyme inhibition upon certain renal and urinary compositional and structural changes which are normally associated with antidiuresis, inhibition was brought about by administration of antiseriam to a purified extract of rat urinary hyaluronidase, raised in rabbits. In rats pretreated with antiserum antidiuretic stimuli failed to elevate the cortico-inedullary solute concentration gradient. This was chiefly a reflection of elevated tissue water content by conparison with similarly treated animals uhich received norDal serum. Following water deprivation for up to forty-ei.;ht hours, urinary volumes v/ere sirnificantly higher in groupsreceiving antiseruia than in [croups receiving noriaal serum. The medullary content of Liucopolysaccharide was estimated by measuring hexosa/aine release on hydrolysis v;ith HOl, It was found that the level decreased during antidiuresis, but pretreatment with antir'Cruia abolished this effect. Pretreatment with antiserum abolished the caluria which noriDally accompanies hydropenia. Excretory levels of calcium between antiserum and normal serum pretreated groups differed significantly throughout the period of water deprivation. In the second twenty-four hour period excretory levels in both groups fell markedly. This was probably due to disturbances in normal body calcium balance resulting from the fall in dietary intalie diiring v.ater deprivation. ( Widening of intercellular spaces in collecting duct epithelium v;as observed in electron Jiicrographs of sections taken froii kidneys of hydropenic rats. Pretreatnent with antiserum decreased both frequency and rize of these spaces. In order to assess the specificity of the enzyme isolated from urine, antiserum to rat testicular hyaluronidase has been raised and its effects.;on the parameters refered topreviously,cxa-uined, Results indicate tnat the testicular ensyme does not have siguificant effect on the renal concentiating process. These findings are consistant 7/ith the view that renal hyaluronidase plays a significant role in the i.;rocess of urinary concentration.
Level: Doctoral
Qualification: Ph.D.
Rights: Copyright © the author. All rights reserved.
Appears in Collections:Theses, Dept. of Cell Physiology and Pharmacology
Leicester Theses

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