Please use this identifier to cite or link to this item:
Title: Increased blood levels of IgG reactive with secreted Streptococcus pyogenes proteins in chronic plaque psoriasis.
Authors: El-Rachkidy, Rana G.
Hales, Jonathan M.
Freestone, Primrose P.E.
Young, Helen
Griffiths, Christopher E.M.
Camp, Richard D.R.
First Published: Jun-2007
Publisher: Nature Publishing Group
Citation: Journal of Investigative Dermatology, 2007, 127(6), pp.1337-1342.
Abstract: A pathogenic role for Streptococcus pyogenes infections in chronic plaque psoriasis is suspected but poorly defined. We separated cellular and supernatant proteins from S pyogenes cultures by high-resolution two-dimensional gel electrophoresis, and used immunoblotting to demonstrate the diversity of serum or plasma IgGs that react with elements of the proteome of this bacterium. We have shown that a substantial proportion of IgG-reactive proteins from cultured S pyogenes are secreted. The total secreted protein fraction, including diverse IgG-binding elements, was subsequently used in an ELISA to measure blood titers of reactive IgG. This ELISA showed that blood samples from patients with chronic plaque psoriasis contained significantly higher titers of reactive IgG than samples from age- and sex-matched healthy controls (p = 0.0009). In contrast, neither a standard assay measuring antistreptolysin O titers nor ELISAs measuring titers of IgG reactive with protein fractions from Staphylococcus aureus and Staphylococcus epidermidis, were able to distinguish between blood samples from the two groups. These findings justify the hypothesis that S pyogenes infections are more important in the pathogenesis of chronic plaque psoriasis than has previously been recognised, and indicate the need for further controlled therapeutic trials of antibacterial measures in this common skin disease.
DOI Link: 10.1038/sj.jid.5700744
Links:; DOI:10.1038/sj.jid.5700744
Version: Post print
Status: Peer reviewed
Type: Article
Rights: Copyright © 2007 The Society for Investigative Dermatology. Deposited with reference to the publisher's archiving policy available on the SHERPA/RoMEO website.
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

Files in This Item:
File Description SizeFormat 
Increased Blood Levels.pdf236.71 kBAdobe PDFView/Open

Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.