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|Title:||A Three-Dimensional Multivariate Image Proccessing Technique for the Analysis of FTIR Spectroscopic Images of Multiple Tissue Sections|
|Authors:||Wood, Bayden R.|
Bambery, Keith R.
Evans, Corey J.
Quinn, Michael A.
|Publisher:||BioMed Central Ltd|
|Citation:||BMC Medical Imaging, 2006, 6:12|
|Abstract:||Background: Three-dimensional (3D) multivariate Fourier Transform Infrared (FTIR) image maps of tissue sections are presented. A villoglandular adenocarcinoma from a cervical biopsy with a number of interesting anatomical features was used as a model system to demonstrate the efficacy of the technique. Methods: Four FTIR images recorded using a focal plane array detector of adjacent tissue sections were stitched together using a MATLAB® routine and placed in a single data matrix for multivariate analysis using Cytospec™. Unsupervised Hierarchical Cluster Analysis (UHCA) was performed simultaneously on all 4 sections and 4 clusters plotted. The four UHCA maps were then stacked together and interpolated with a box function using SCIRun software. Results: The resultant 3D-images can be rotated in three-dimensions, sliced and made semi-transparent to view the internal structure of the tissue block. A number of anatomical and histopathological features including connective tissue, red blood cells, inflammatory exudate and glandular cells could be identified in the cluster maps and correlated with Hematoxylin & Eosin stained sections. The mean extracted spectra from individual clusters provide macromolecular information on tissue components. Conclusion: 3D-multivariate imaging provides a new avenue to study the shape and penetration of important anatomical and histopathological features based on the underlying macromolecular chemistry and therefore has clear potential in biology and medicine.|
|Rights:||Copyright © 2006 Wood et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.|
|Appears in Collections:||Published Articles, Dept. of Chemistry|
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|10.1186_1471-2342-6-12.pdf||Post-review (final submitted)||1.64 MB||Adobe PDF||View/Open|
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