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Title: Natural growth regulators in suspension cultures of Acer pseudoplatanus, l.
Authors: Mackenzie, Ian A.
Award date: 1970
Presented at: University of Leicester
Abstract: Sycamore cell auspension cultures in a synthetic mediua have been shown to release ethylene. The rate of release has been monitored in relation to the growth of the cella. Evidence is presented that 2, 4, -dichlorophenoxy acetic acid enhancea ethylene production independently of its effects on cultures growth. Under standard conditions of culture (250 ml Erlenmeyer flask closed with aluminium foil, and containing 70 ml cell suspension) the concentration of ethylene in the gas phase of the cultures rises above 10 ppm. No evidence was obtained that this ethylene is inhibitory to culture growth, or that a critical level of ethylene is necessary to initiate cell division in cultures at a critically low cell density. The low rate of ethylene release by stationary phase cultures is temporarily enhanced by the addition of various solutes, and further depressed by dilution with water. Cytokinin activity has been detected in ethanolic extracts of sycamore cells, hydrolysates of sRNA from these cells, and in medium which has supported their growth. The possible identities of these compounds is discussed. Changes that occur in the amounts of certain cytokinins present in the cells, have been monitored in relation to the growth cycle of the cultures. It has been demonstrated that under certain cultural conditions the growth of the sycamore calls is enhanced by exogenously supplied oytokinins. The relative activities of various natural and synthetic cytokinins have been tested under these conditions.
Level: Doctoral
Qualification: Ph.D.
Rights: Copyright © the author. All rights reserved.
Appears in Collections:Theses, Dept. of Biology
Leicester Theses

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