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|Title:||Evidence for Nociceptin/Orphanin FQ (NOP) but not µ (MOP), δ (DOP) or κ (KOP) opioid receptor mRNA in whole human blood|
|Authors:||Lambert, David G.|
Thompson, J. P.
|Publisher:||Oxford University Press (OUP)|
|Citation:||British Journal of Anaesthesia (2016) 116 (3), pp. 423-429.|
|Abstract:||Background While it is well known that opioids depress the immune system, the site(s) of action for this depression is highly controversial. Immune modulation could occur directly at the immune cell or centrally via the hypothalamic-pituitary-adrenal axis. In a number of studies using individual enriched immune cell populations we have failed to detect classical µ (MOP), δ (DOP) and κ (KOP) receptors. The non-classical nociceptin/orphanin FQ (N/OFQ) receptor (NOP) is expressed on all cells examined thus far. Our hypothesis was that immune cells do not express classical opioid receptors and that using whole blood would definitively answer this question. Methods Whole blood (containing all immune cell types) was incubated with opioids (morphine and fentanyl) commonly encountered in anaesthesia and with agents mimicking sepsis [lipopolysaccharide (LPS) and peptidoglycan G (PepG)]. Opioid receptor mRNA expression was assessed by endpoint polymerase chain reaction (PCR) with gel visualisation and quantitative PCR. Results Classical MOP, DOP, and KOP receptors were not detected in any of the samples tested either at rest or when challenged with opioids, LPS or PepG. Commercial primers for DOP did not perform well in quantitative PCR, so the absence of expression was confirmed using a traditional gel-based approach. NOP receptors were detected in all samples; expression was unaffected by opioids and reduced by LPS/PepG combinations. Conclusions Classical opioid receptors are not expressed on circulating immune cells.|
|Rights:||Copyright © 2016 the authors. Published by Oxford University Press on behalf of the British Journal of Anaesthesia. This is a pre-copyedited, author-produced PDF of an article accepted for publication in British Journal of Anaesthesia following peer review. The version of record British Journal of Anaesthesia (2016) 116 (3), pp. 423-429 is available online at: dx.doi.org/10.1093/bja/aev540|
|Description:||The file associated with this record is under a 12-month embargo from publication in accordance with the publisher's self-archiving policy. The full text may be available through the publisher links provided above.|
|Appears in Collections:||Published Articles, Dept. of Cardiovascular Sciences|
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|BJA-2015-00829-RM142 REVISED.docx||Post-review (final submitted)||3.75 MB||Unknown||View/Open|
|BJA-2015-00829-RM142 REVISED.pdf||Post-review (final submitted)||1.03 MB||Adobe PDF||View/Open|
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