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|Title:||Distinct and complementary roles for α and β isoenzymes of protein kinase C in mediating vasoconstrictor responses to acutely elevated glucose|
Sims, Mark W.
Challiss, R. A. J.
Squire, Iain B.
|Citation:||British Journal of Pharmacology, 2016, 173(5), pp. 870-887|
|Abstract:||Background and Purpose: We investigate the hypothesis that elevated glucose increases contractile responses in vascular smooth muscle, and that this enhanced constriction occurs due to the glucose-induced protein kinase C (PKC)-dependent inhibition of voltage-gated potassium channels. Experimental Approach: Patch-clamp electrophysiology in rat isolated mesenteric arterial myocytes was performed to investigate the glucose-induced inhibition of voltage-gated potassium (Kv) current. To determine the effects of glucose in whole vessel wire myography was performed in rat mesenteric, porcine coronary and human internal mammary arteries. Key Results: Glucose-induced inhibition of Kv was PKC-dependent and could be pharmacologically dissected using PKC isoenzyme-specific inhibitors to reveal a PKCβ-dependent component of Kv inhibition dominating between 0 and 10 mM glucose with an additional PKCα-dependent component becoming evident at concentrations greater than 10 mM. These findings were supported using wire myography in all artery types used, where contractile responses to vessel depolarization and vasoconstrictors were enhanced by increasing bathing glucose concentration, again with evidence for distinct and complementary PKCα/PKCβ-mediated components. Conclusions and Implications: Our study provides compelling evidence that glucose-induced PKCα/PKCβ-mediated inhibition of Kv current in vascular smooth muscle causes an enhanced constrictor response. Inhibition of Kv current causes a significant depolarization of vascular myocytes leading to marked vasoconstriction. The PKC-dependence of this enhanced constrictor response may present a potential therapeutic target for improving microvascular perfusion following percutaneous coronary intervention following myocardial infarction in hyperglycaemic patients.|
|Rights:||Copyright © The British Pharmacological Society, 2016. All rights reserved. This is the peer reviewed version of the following article: Jackson, R., Brennan, S., Fielding, P., Sims, M. W., Challiss, RA. J., Adlam, D., Squire, I. B., and Rainbow, R. D. (2016) Distinct and complementary roles for α and β isoenzymes of PKC in mediating vasoconstrictor responses to acutely elevated glucose. British Journal of Pharmacology, 173: 870–887, which has been published in final form at dx.doi.org/10.1111/bph.13399. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.|
|Description:||The file associated with this record is under a 12-month embargo from publication in accordance with the publisher's self-archiving policy, available at http://olabout.wiley.com/WileyCDA/Section/id-820227.html. The full text may be available through the publisher links provided above.|
|Appears in Collections:||Published Articles, Dept. of Cardiovascular Sciences|
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|Jackson et al Accepted version for Iris.docx||Post-review (final submitted)||3.02 MB||Unknown||View/Open|
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