Please use this identifier to cite or link to this item:
Title: Stepwise visualization of membrane pore formation by suilysin, a bacterial cholesterol-dependent cytolysin
Authors: Leung, C.
Dudkina, N. V.
Lukoyanova, N.
Hodel, A. W.
Farabella, I.
Pandurangan, A. P.
Jahan, N.
Pires Damaso, M.
Osmanović, D. J.
Reboul, C. F.
Dunstone, M. A.
Andrew, Peter William
Lonnen, R.
Topf, M.
Saibil, H. R.
Hoogenboom, B. W.
First Published: 2-Dec-2014
Publisher: eLife Sciences Publications
Citation: Elife, 2014, 3, e04247
Abstract: Membrane attack complex/perforin/cholesterol-dependent cytolysin (MACPF/CDC) proteins constitute a major superfamily of pore-forming proteins that act as bacterial virulence factors and effectors in immune defence. Upon binding to the membrane, they convert from the soluble monomeric form to oligomeric, membrane-inserted pores. Using real-time atomic force microscopy (AFM), electron microscopy (EM), and atomic structure fitting, we have mapped the structure and assembly pathways of a bacterial CDC in unprecedented detail and accuracy, focussing on suilysin from Streptococcus suis. We show that suilysin assembly is a noncooperative process that is terminated before the protein inserts into the membrane. The resulting ring-shaped pores and kinetically trapped arc-shaped assemblies are all seen to perforate the membrane, as also visible by the ejection of its lipids. Membrane insertion requires a concerted conformational change of the monomeric subunits, with a marked expansion in pore diameter due to large changes in subunit structure and packing.
DOI Link: 10.7554/eLife.04247
eISSN: 2050-084X
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2014, Leung et al This article is distributed under the terms of the Creative Commons Attribution License ( ), which permits unrestricted use and redistribution provided that the original author and source are credited.
Description: This publication has a correction, available at
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

Files in This Item:
File Description SizeFormat 
Stepwise visualization of membrane pore formation by suilysin, a bacterial cholesterol-dependent cytolysin..pdfPublished (publisher PDF)2.71 MBAdobe PDFView/Open

Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.