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http://hdl.handle.net/2381/36364
Title: | Solution structure of the YTH domain in complex with N6-methyladenosine RNA: a reader of methylated RNA |
Authors: | Theler, D. Dominguez, Cyril Blatter, M. Boudet, J. Allain, F. H. |
First Published: | 11-Nov-2014 |
Publisher: | Oxford University Press (OUP) |
Citation: | Nucleic Acids Research, 2014, 42 (22), pp. 13911-13919 |
Abstract: | N(6)A methylation is the most abundant RNA modification occurring within messenger RNA. Impairment of methylase or demethylase functions are associated with severe phenotypes and diseases in several organisms. Beside writer and eraser enzymes of this dynamic RNA epigenetic modification, reader proteins that recognize this modification are involved in numerous cellular processes. Although the precise characterization of these reader proteins remains unknown, preliminary data showed that most potential reader proteins contained a conserved YT521-B homology (YTH) domain. Here we define the YTH domain of rat YT521-B as a N(6)-methylated adenosine reader domain and report its solution structure in complex with a N(6)-methylated RNA. The structure reveals a binding preference for NGANNN RNA hexamer and a deep hydrophobic cleft for m(6)A recognition. These findings establish a molecular function for YTH domains as m(6)A reader domains and should guide further studies into the biological functions of YTH-containing proteins in m(6)A recognition. |
DOI Link: | 10.1093/nar/gku1116 |
ISSN: | 0305-1048 |
eISSN: | 1362-4962 |
Links: | http://nar.oxfordjournals.org/content/42/22/13911 http://hdl.handle.net/2381/36364 |
Version: | Publisher Version |
Status: | Peer-reviewed |
Type: | Journal Article |
Rights: | Copyright © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
Appears in Collections: | Published Articles, College of Medicine, Biological Sciences and Psychology |
Files in This Item:
File | Description | Size | Format | |
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Solution structure of the YTH domain in complex with N6-methyladenosine RNA: a reader of methylated RNA..pdf | Published (publisher PDF) | 2.79 MB | Adobe PDF | View/Open |
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