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|Title:||Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library|
Rees, J. S.
Armean, I. M.
Magbanua, J. P.
Armstrong, J. D.
Phillips, R. G.
Lilley, K. S.
St Johnston, D.
|Publisher:||Company of Biologists: Development|
|Citation:||Development 2014 141: 3994-400|
|Abstract:||Although we now have a wealth of information on the transcription patterns of all the genes in the Drosophila genome, much less is known about the properties of the encoded proteins. To provide information on the expression patterns and subcellular localisations of many proteins in parallel, we have performed a large-scale protein trap screen using a hybrid piggyBac vector carrying an artificial exon encoding yellow fluorescent protein (YFP) and protein affinity tags. From screening 41 million embryos, we recovered 616 verified independent YFP-positive lines representing protein traps in 374 genes, two-thirds of which had not been tagged in previous P element protein trap screens. Over 20 different research groups then characterized the expression patterns of the tagged proteins in a variety of tissues and at several developmental stages. In parallel, we purified many of the tagged proteins from embryos using the affinity tags and identified co-purifying proteins by mass spectrometry. The fly stocks are publicly available through the Kyoto Drosophila Genetics Resource Center. All our data are available via an open access database (Flannotator), which provides comprehensive information on the expression patterns, subcellular localisations and in vivo interaction partners of the trapped proteins. Our resource substantially increases the number of available protein traps in Drosophila and identifies new markers for cellular organelles and structures.|
|Rights:||© 2014. Published by The Company of Biologists Ltd This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.|
|Description:||Supplementary material available online at http://dev.biologists.org/lookup/suppl/doi:10.1242/dev.111054/-/DC1|
|Appears in Collections:||Published Articles, Dept. of Genetics|
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