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|Title:||Oleoyl coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in mycobacteria|
Waddell, S. J.
Sarybaeva, Asel A.
Young, D. I.
Young, R. M.
Andrew, Peter William
Butcher, P. D.
Mukamolova, Galina V.
|Publisher:||American Society for Biochemistry and Molecular Biology|
|Citation:||Journal of Biological Chemistry , 2014, 289 (36), pp. 25241-25249|
|Abstract:||We have recently shown that RaaS (regulator of antimicrobial-assisted survival), encoded by Rv1219c in Mycobacterium tuberculosis and by bcg_1279c in Mycobacterium bovis bacillus Calmette-Guérin, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here, we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and, to lesser extent, palmitoyl-CoA) modulate RaaS binding to DNA and expression of the downstream genes that encode ATP-dependent efflux pumps. Moreover, exogenously added oleic acid influences RaaS-mediated mycobacterial improvement of survival and expression of the RaaS regulon. Our data suggest that long chain acyl-CoA derivatives serve as biological indicators of the bacterial metabolic state. Dysregulation of efflux pumps can be used to eliminate non-growing mycobacteria.|
|Rights:||Copyright © 2014 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version full access. Creative Commons Attribution Unported License ( http://creativecommons.org/licenses/by/3.0/ ) applies to Author Choice Articles|
|Appears in Collections:||Published Articles, Dept. of Infection, Immunity and Inflammation|
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|J. Biol. Chem.-2014-Turapov-25241-9.pdf||Published (publisher PDF)||2.36 MB||Adobe PDF||View/Open|
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