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Title: Oleoyl coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in mycobacteria
Authors: Turapov, Obolbek
Waddell, S. J.
Burke, Bernard
Glenn, Sarah
Sarybaeva, Asel A.
Tudo, G.
Labesse, G.
Young, D. I.
Young, R. M.
Andrew, Peter William
Butcher, P. D.
Cohen-Gonsaud, M.
Mukamolova, Galina V.
First Published: 10-Jul-2014
Publisher: American Society for Biochemistry and Molecular Biology
Citation: Journal of Biological Chemistry , 2014, 289 (36), pp. 25241-25249
Abstract: We have recently shown that RaaS (regulator of antimicrobial-assisted survival), encoded by Rv1219c in Mycobacterium tuberculosis and by bcg_1279c in Mycobacterium bovis bacillus Calmette-Guérin, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here, we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and, to lesser extent, palmitoyl-CoA) modulate RaaS binding to DNA and expression of the downstream genes that encode ATP-dependent efflux pumps. Moreover, exogenously added oleic acid influences RaaS-mediated mycobacterial improvement of survival and expression of the RaaS regulon. Our data suggest that long chain acyl-CoA derivatives serve as biological indicators of the bacterial metabolic state. Dysregulation of efflux pumps can be used to eliminate non-growing mycobacteria.
DOI Link: 10.1074/jbc.M114.577338
ISSN: 0021-9258
eISSN: 1083-351X
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2014 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version full access. Creative Commons Attribution Unported License ( ) applies to Author Choice Articles
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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