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Title: Genome-wide protein QTL mapping identifies human plasma kallikrein as a post-translational regulator of serum uPAR levels.
Authors: Portelli, M. A.
Siedlinski, M.
Stewart, C. E.
Postma, D. S.
Nieuwenhuis, M. A.
Vonk, J. M.
Nurnberg, P.
Altmuller, J.
Moffatt, M. F.
Wardlaw, Andrew John
Parker, S. G.
Connolly, M. J.
Koppelman, G. H.
Sayers, I.
First Published: 18-Nov-2013
Publisher: Federation of American Society of Experimental Biology (FASEB)
Citation: FASEB Journal, 2014, 28 (2), pp. 923-934
Abstract: The soluble cleaved urokinase plasminogen activator receptor (scuPAR) is a circulating protein detected in multiple diseases, including various cancers, cardiovascular disease, and kidney disease, where elevated levels of scuPAR have been associated with worsening prognosis and increased disease aggressiveness. We aimed to identify novel genetic and biomolecular mechanisms regulating scuPAR levels. Elevated serum scuPAR levels were identified in asthma (n=514) and chronic obstructive pulmonary disease (COPD; n=219) cohorts when compared to controls (n=96). In these cohorts, a genome-wide association study of serum scuPAR levels identified a human plasma kallikrein gene (KLKB1) promoter polymorphism (rs4253238) associated with serum scuPAR levels in a control/asthma population (P=1.17 × 10(-7)), which was also observed in a COPD population (combined P=5.04 × 10(-12)). Using a fluorescent assay, we demonstrated that serum KLKB1 enzymatic activity was driven by rs4253238 and is inverse to scuPAR levels. Biochemical analysis identified that KLKB1 cleaves scuPAR and negates scuPAR's effects on primary human bronchial epithelial cells (HBECs) in vitro. Chymotrypsin was used as a proproteolytic control, while basal HBECs were used as a control to define scuPAR-driven effects. In summary, we reveal a novel post-translational regulatory mechanism for scuPAR using a hypothesis-free approach with implications for multiple human diseases.
DOI Link: 10.1096/fj.13-240879
ISSN: 0892-6638
eISSN: 1530-6860
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2013, FASEB. This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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