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|Title:||The Placental Variant of Human Growth Hormone Reduces Maternal Insulin Sensitivity in a Dose-Dependent Manner in C57BL/6J Mice.|
Vickers, M. H.
Stanley, J. L.
Ponnampalam, A. P.
Baker, Philip Newton
Perry, J. K.
|Citation:||Endocrinology, 2016, 157 (3), pp. 1175-1186|
|Abstract:||The human placental GH variant (GH-V) is secreted continuously from the syncytiotrophoblast layer of the placenta during pregnancy and is thought to play a key role in the maternal adaptation to pregnancy. Maternal GH-V concentrations are closely related to fetal growth in humans. GH-V has also been proposed as a potential candidate to mediate insulin resistance observed later in pregnancy. To determine the effect of maternal GH-V administration on maternal and fetal growth and metabolic outcomes during pregnancy, we examined the dose-response relationship for GH-V administration in a mouse model of normal pregnancy. Pregnant C57BL/6J mice were randomized to receive vehicle or GH-V (0.25, 1, 2, or 5 mg/kg · d) by osmotic pump from gestational days 12.5 to 18.5. Fetal linear growth was slightly reduced in the 5 mg/kg dose compared with vehicle and the 0.25 mg/kg groups, respectively, whereas placental weight was not affected. GH-V treatment did not affect maternal body weights or food intake. However, treatment with 5 mg/kg · d significantly increased maternal fasting plasma insulin concentrations with impaired insulin sensitivity observed at day 18.5 as assessed by homeostasis model assessment. At 5 mg/kg · d, there was also an increase in maternal hepatic GH receptor/binding protein (Ghr/Ghbp) and IGF binding protein 3 (Igfbp3) mRNA levels, but GH-V did not alter maternal plasma IGF-1 concentrations or hepatic Igf-1 mRNA expression. Our findings suggest that at higher doses, GH-V treatment can cause hyperinsulinemia and is a likely mediator of the insulin resistance associated with late pregnancy.|
|Rights:||Copyright © 2016 by the Endocrine Society. Deposited with reference to the publisher’s archiving policy available on the SHERPA/RoMEO website.|
|Appears in Collections:||Published Articles, College of Medicine, Biological Sciences and Psychology|
Files in This Item:
|Manusript 13-7-15 st..doc||Post-review (final submitted)||258 kB||Microsoft Word||View/Open|
|Supplementary Figure 1.pptx||Post-review (final submitted)||800.72 kB||Unknown||View/Open|
|Table 13-7-15 st.docx||Post-review (final submitted)||22.65 kB||Unknown||View/Open|
|Manusript 13-7-15 st..pdf||Post-review (final submitted)||403.02 kB||Adobe PDF||View/Open|
|Supplementary Figure 1.pdf||Post-review (final submitted)||230.19 kB||Adobe PDF||View/Open|
|Table 13-7-15 st.pdf||Post-review (final submitted)||107.47 kB||Adobe PDF||View/Open|
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