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Title: Electron transfer dissociation of native peptides facilitates enhanced identification of urinary peptides
Authors: Hart, S. R.
Kenny, L. C.
Myers, J. E.
Baker, Philip Newton
First Published: 3-Sep-2015
Publisher: Elsevier
Citation: International Journal Of Mass Spectrometry, 2015, 391, pp. 41-46 (6)
Abstract: Urine as a biofluid is commonly used in clinical diagnostics, including those performed during pregnancy. Urine is a rich source of polypeptides and polypeptidic protein degradation products, which have been filtered from blood plasma, thus urine has potential as a source for novel clinical diagnostics in disease. In this study, we examine the urinary peptidome from normal healthy women during pregnancy, and demonstrate ready observation of large polypeptide. We utilise the dissociation method, electron transfer dissociation (ETD) to increase the identification rate of the peptides present within these samples, as the polypeptide species observed in these samples are large and highly charged. An increase in the number of peptides whose identities could be ascribed using routine database searching methods was enabled via the use of ETD.
DOI Link: 10.1016/j.ijms.2015.08.025
ISSN: 1387-3806
Version: Post-print
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2015 Elsevier B.V. All rights reserved. This manuscript version is made available after the end of the embargo period under the CC-BY-NC-ND 4.0 license 
Description: The file associated with this record is under a 24-month embargo from publication in accordance with the publisher's self-archiving policy. The full text may be available through the publisher links provided above.
Appears in Collections:Published Articles, College of Medicine, Biological Sciences and Psychology

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