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Title: Full Genome Characterization of Human Influenza A/H3N2 Isolates from Asian Countries Reveals a Rare Amantadine Resistance-Conferring Mutation and Novel PB1-F2 Polymorphisms
Authors: Zaraket, H.
Kondo, H.
Hibino, A.
Yagami, R.
Odagiri, T.
Takemae, N.
Tsunekuni, R.
Saito, T.
Japanese Influenza Collaborative Study Group
Myint, Y. Y.
Kyaw, Y.
Oo, K. Y.
Tin, H. H.
Lin, N.
Anh, N. P.
Hang, N. L. K.
Mai, L. Q.
Hassan, M. R.
Shobugawa, Y.
Tang, Julian
Dbaibo, G.
Saito, R.
First Published: 7-Mar-2016
Publisher: Frontiers Media
Citation: Frontiers in Microbiology 2016; 7: 262.
Abstract: Influenza A viruses evolve at a high rate requiring continuous monitoring to maintain the efficacy of vaccines and antiviral drugs. We performed next generation sequencing analysis of 100 influenza A/H3N2 isolates collected in four Asian countries (Japan, Lebanon, Myanmar, and Vietnam) during 2012–2015. Phylogenetic analysis revealed several reassortment events leading to the circulation of multiple clades within the same season. This was particularly evident during the 2013 and 2013/2014 seasons. Importantly, our data showed that certain lineages appeared to be fitter and were able to persist into the following season. The majority of A/H3N2 viruses continued to harbor the M2-S31N mutation conferring amantadine-resistance. In addition, an S31D mutation in the M2-protein, conferring a similar level of resistance as the S31N mutation, was detected in three isolates obtained in Japan during the 2014/2015 season. None of the isolates possessed the NA-H274Y mutation conferring oseltamivir-resistance, though a few isolates were found to contain mutations at the catalytic residue 151 (D151A/G/N or V) of the NA protein. These variations did not alter the susceptibility to neuraminidase inhibitors and were not detected in the original clinical specimens, suggesting that they had been acquired during their passage in MDCK cells. Novel polymorphisms were detected in the PB1-F2 open-reading frame resulting in truncations in the protein of 24–34 aminoacids in length. Thus, this study has demonstrated the utility of monitoring the full genome of influenza viruses to allow the detection of the potentially fittest lineages. This enhances our ability to predict the strain(s) most likely to persist into the following seasons and predict the potential degree of vaccine match or mismatch with the seasonal influenza season for that year. This will enable the public health and clinical teams to prepare for any related healthcare burden, depending on whether the vaccine match is predicted to be good or poor for that season.
DOI Link: 10.3389/fmicb.2016.00262
eISSN: 1664-302X
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2016 Zaraket, Kondo, Hibino, Yagami, Odagiri, Takemae, Tsunekuni, Saito, Japanese Influenza Collaborative Study Group, Myint, Kyaw, Oo, Tin, Lin, Anh, Hang, Mai, Hassan, Shobugawa, Tang, Dbaibo and Saito. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Description: The Supplementary Material for this article can be found online at:
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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