Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/37405
Title: Replicational organization of three weakly expressed loci in Physarum polycephalum
Authors: Maric, C.
Swanston, E.
Bailey, Juliet Anne
Pierron, G.
First Published: 1-Jun-2002
Publisher: Oxford University Press (OUP)
Citation: Nucleic Acids Research, 2002, 30 (11), pp. 2261-2269
Abstract: We previously mapped early-activated replication origins in the promoter regions of five abundantly transcribed genes in the slime mold Physarum polycephalum. This physical linkage between origins and genes is congruent with the preferential early replication of the active genes in mammalian cells. To determine how general this replicational organization is in the synchronous plasmodium of Physarum, we analyzed the replication of three weakly expressed genes. Bromodeoxyuridine (BrdUrd) density-shift and gene dosage experiments indicated that the redB (regulated in development) and redE genes replicate early, whereas redA replicates in mid-S phase. Bi-dimensional gel electrophoresis revealed that redA coincides with an origin that appears to be activated within a large temporal window in S phase so that the replication of the gene is not well defined temporally. The early replication of the redB and redE genes is due to the simultaneous activation of flanking origins at the onset of S phase. As a result, these two genes correspond to termination sites of DNA replication. Our data demonstrate that not all the Physarum promoters are preferred sites of initiation but, so far, all the expressed genes analyzed in detail either coincide with a replication origin or are embedded into a cluster of early firing replicons.
DOI Link: 10.1093/nar/30.11.2261
ISSN: 0305-1048
eISSN: 1362-4962
Links: https://nar.oxfordjournals.org/content/30/11/2261.abs
http://hdl.handle.net/2381/37405
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2002 Oxford University Press. The file associated with this record is distributed under the Creative Commons “Attribution Non-Commercial No Derivatives” licence, further details of which can be found via the following link: http://creativecommons.org/licenses/by-nc-nd/4.0/
Appears in Collections:Published Articles, Research Support Office

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