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Title: Multilocus analysis of introgression between two sand fly vectors of leishmaniasis
Authors: Mazzoni, C. J.
Araki, A. S.
Ferreira, G. E. M.
Azevedo, Renata V. D. M.
Barbujani, G.
Peixoto, A. A.
First Published: 12-May-2008
Publisher: BioMed Central
Citation: BMC Evolutionary Biology 2008 8:141
Abstract: Background The phlebotomine sand flies (Diptera:Psychodidae) Lutzomyia (Nyssomyia) intermedia Lutz & Neiva 1912 and Lutzomyia (Nyssomyia) whitmani Antunes & Coutinho 1932 are two very closely related species and important vectors of American cutaneous leishmaniasis. Two single-locus studies have revealed evidence for introgression between the two species in both mitochondrial and nuclear genomes. These findings have prompted the development of a multilocus approach to investigate in more detail the genetic exchanges between the two species. Results We analyzed ten nuclear loci using the "isolation with migration" model implemented in the IM program, finding evidence for introgression from L. intermedia towards L. whitmani in three loci. These results confirm that introgression is occurring between the two species and suggest variation in the effects of gene flow among the different regions of the genome. Conclusion The demonstration that these two vectors are not fully reproductively isolated might have important epidemiological consequences as these species could be exchanging genes controlling aspects of their vectorial capacity.
DOI Link: 10.1186/1471-2148-8-141
ISSN: 1471-2148
eISSN: 1471-2148
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: © 2008 Mazzoni et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Description: Additional file 1 Supplemental Table – Tests of neutrality. The table presents the results of neutrality tests Fu and Li's D* and F*, Fu's FS and Ramos-Onsins and Rozas' R2. [ 2148-8-141-S1.doc] Additional file 2 Genealogies of RpL17A and zetacop whole fragments. The figure shows trees of RpL17A and zetacop sequences (whole fragments) of L. intermedia (blue circles) and L. whitmani (red squares). The trees were estimated using the neighbor-joining method, Kimura-2-parameters distance and 1000 bootstrap replicates, and rooted using the middle-point between the two most distant sequences. [ 2148-8-141-S2.pdf] Additional file 3 Haplotype networks of RpL17A and zetacop whole fragments. The figure shows minimum spanning trees of RpL17A and zetacop sequences (whole fragments) of L. intermedia (blue) and L. whitmani (red). The circles are proportional to the haplotype frequencies and the black and grey lines connecting the haplotypes represent the number of mutational steps. [ 2148-8-141-S3.pdf]
Appears in Collections:Published Articles, Dept. of Genetics

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