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|Title:||Extracellular vesicles released by CD40/IL-4 stimulated chronic lymphocytic leukemia cells confer altered functional properties to CD4+ T cells|
|Authors:||Smallwood, Dawn T.|
Ambrose, Ashley R.
De Rossi, Giulia
Ramsay, Alan G.
Wagner, Simon D.
|Publisher:||American Society of Hematology|
|Citation:||Blood, 2016, 128 (4), pp. 542-552|
|Abstract:||The complex interplay between cancer cells, stromal cells and immune cells in the tumor microenvironment (TME) regulates tumorigenesis and provides emerging targets for immunotherapies. Crosstalk between CD4(+) T cells and proliferating chronic lymphocytic leukemia (CLL) tumor B cells occurs within lymphoid tissue pseudo-follicles and investigating these interactions is essential to understand both disease pathogenesis and the effects of immunotherapy. Tumor-derived extracellular vesicle (EV) shedding is emerging as an important mode of intercellular communication in the TME. In order to characterize tumor EVs released in response to T cell-derived TME signals, we performed microRNA (miR) profiling of EVs released from CLL cells stimulated with CD40 and IL-4. Our results reveal an enrichment of specific cellular miRNAs including miR-363 within EVs derived from CD40/IL-4 stimulated CLL cells compared to parental cell miRNA content and control EVs from unstimulated CLL cells. We demonstrate that autologous patient CD4(+) T cells internalize CLL-EVs containing miR-363 that targets the immunomodulatory molecule CD69. We further reveal that autologous CD4(+) T cells that are exposed to EVs from CD40/IL-4 stimulated CLL cells exhibit enhanced migration, immunological synapse signaling and interactions with tumor cells. Knockdown of miR-363 in CLL cells prior to CD40/IL-4 stimulation, prevented the ability of CLL-EVs to induce increased synapse signaling and confer altered functional properties to CD4(+) T cells. Taken together, these data reveal a novel role for CLL-EVs in modifying T cell function that highlights unanticipated complexity of intercellular communication that may have implications for bidirectional CD4(+) T-cell:tumor interactions within the TME.|
|Rights:||Copyright © the authors, 2016. This version is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/ ), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.|
|Appears in Collections:||Published Articles, Dept. of Cancer Studies and Molecular Medicine|
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