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Title: Genomic and proteomic characterization of two novel siphovirus infecting the sedentary facultative epibiont cyanobacterium Acaryochloris marina
Authors: Chan, Yi-Wah
Millard, Andrew D.
Wheatley, Peter J.
Holmes, Antony B.
Mohr, Remus
Whitworth, Anna L.
Mann, Nicholas H.
Larkum, Anthony W. D.
Hess, Wolfgang R.
Scanlan, David J.
Clokie, Martha R. J.
First Published: 11-Nov-2015
Publisher: Wiley on behalf of Society for Applied Microbiology (SfAM)
Citation: Environmental Microbiology, 2015, 17 (11), pp. 4239-4252
Abstract: Summary: Acaryochloris marina is a symbiotic species of cyanobacteria that is capable of utilizing far-red light. We report the characterization of the phages A-HIS1 and A-HIS2, capable of infecting Acaryochloris. Morphological characterization of these phages places them in the family Siphoviridae. However, molecular characterization reveals that they do not show genetic similarity with any known siphoviruses. While the phages do show synteny between each other, the nucleotide identity between the phages is low at 45–67%, suggesting they diverged from each other some time ago. The greatest number of genes shared with another phage (a myovirus infecting marine Synechococcus) was four. Unlike most other cyanophages and in common with the Siphoviridae infecting Synechococcus, no photosynthesis-related genes were found in the genome. CRISPR (clustered regularly interspaced short palindromic repeats) spacers from the host Acaryochloris had partial matches to sequences found within the phages, which is the first time CRISPRs have been reported in a cyanobacterial/cyanophage system. The phages also encode a homologue of the proteobacterial RNase T. The potential function of RNase T in the mark-up or digestion of crRNA hints at a novel mechanism for evading the host CRISPR system.
DOI Link: 10.1111/1462-2920.12735
ISSN: 1462-2912
eISSN: 1462-2920
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © the authors, 2015. This is an open-access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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