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Title: Assessing Illumina technology for the high-throughput sequencing of bacteriophage genomes
Authors: Rihtman, Branko
Meaden, Sean
Clokie, Martha R.J.
Koskella, Britt
Millard, Andrew D.
First Published: 1-Jun-2016
Publisher: PeerJ
Citation: PeerJ, 2016, 4:e2055
Abstract: Bacteriophages are the most abundant biological entities on the planet, playing crucial roles in the shaping of bacterial populations. Phages have smaller genomes than their bacterial hosts, yet there are currently fewer fully sequenced phage than bacterial genomes. We assessed the suitability of Illumina technology for high-throughput sequencing and subsequent assembly of phage genomes. In silico datasets reveal that 30× coverage is sufficient to correctly assemble the complete genome of ~98.5% of known phages, with experimental data confirming that the majority of phage genomes can be assembled at 30× coverage. Furthermore, in silico data demonstrate it is possible to co-sequence multiple phages from different hosts, without introducing assembly errors.
DOI Link: 10.7717/peerj.2055
eISSN: 2167-8359
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © the authors, 2016. This is an open-access article distributed under the terms of the Creative Commons Attribution License ( ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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