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Title: MegaPlex PCR: a strategy for multiplex amplification
Authors: Meuzelaar, Linda Strömqvist
Lancaster, Owen
Pasche, J. Paul
Kopal, Guido
Brookes, Anthony J.
First Published: 16-Sep-2007
Publisher: Nature Publishing Group
Citation: Nature Methods, 2007, 4, pp.835-857
Abstract: ‘MegaPlex PCR’ is a robust technology for highly multiplexed amplification of specific DNA sequences. It uses target-specific pairs of PCR primers that are physically separated by surface immobilization. Initial surface-based amplification cycles are then coupled to efficient solution-phase PCR using one common primer pair. We demonstrate this method by coamplifying and genotyping 75 unselected human single-nucleotide polymorphism (SNP) loci.
Type: Article
Description: This is the authors' final draft of the paper published as Nature Methods, 2007, 4, pp.835-837. The definitive published version is available from, doi:10.1038/nmeth1091
Appears in Collections:Published Articles, Dept. of Genetics

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