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|Title:||MegaPlex PCR: a strategy for multiplex amplification|
|Authors:||Meuzelaar, Linda Strömqvist|
Pasche, J. Paul
Brookes, Anthony J.
|Publisher:||Nature Publishing Group|
|Citation:||Nature Methods, 2007, 4, pp.835-857|
|Abstract:||‘MegaPlex PCR’ is a robust technology for highly multiplexed amplification of specific DNA sequences. It uses target-specific pairs of PCR primers that are physically separated by surface immobilization. Initial surface-based amplification cycles are then coupled to efficient solution-phase PCR using one common primer pair. We demonstrate this method by coamplifying and genotyping 75 unselected human single-nucleotide polymorphism (SNP) loci.|
|Description:||This is the authors' final draft of the paper published as Nature Methods, 2007, 4, pp.835-837. The definitive published version is available from http://www.nature.com/nmeth/, doi:10.1038/nmeth1091|
|Appears in Collections:||Published Articles, Dept. of Genetics|
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