Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/38471
Title: Fluorescence-based assay as a new screening tool for toxic chemicals
Authors: Moczko, E.
Mirkes, Evgeny M.
Cáceres, C.
Gorban, Alexander N.
Piletsky, Sergey
First Published: 22-Sep-2016
Publisher: Nature Publishing Group
Citation: Scientific Reports, 2016, 6:33922
Abstract: Our study involves development of fluorescent cell-based diagnostic assay as a new approach in high-throughput screening method. This highly sensitive optical assay operates similarly to e-noses and e-tongues which combine semi-specific sensors and multivariate data analysis for monitoring biochemical processes. The optical assay consists of a mixture of environmental-sensitive fluorescent dyes and human skin cells that generate fluorescence spectra patterns distinctive for particular physico-chemical and physiological conditions. Using chemometric techniques the optical signal is processed providing qualitative information about analytical characteristics of the samples. This integrated approach has been successfully applied (with sensitivity of 93% and specificity of 97%) in assessing whether particular chemical agents are irritating or not for human skin. It has several advantages compared with traditional biochemical or biological assays and can impact the new way of high-throughput screening and understanding cell activity. It also can provide reliable and reproducible method for assessing a risk of exposing people to different harmful substances, identification active compounds in toxicity screening and safety assessment of drugs, cosmetic or their specific ingredients.
DOI Link: 10.1038/srep33922
ISSN: 2045-2322
eISSN: 2045-2322
Links: http://www.nature.com/articles/srep33922
http://hdl.handle.net/2381/38471
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Description: Supplementary information accompanies this paper at http://www.nature.com/srep
Appears in Collections:Published Articles, Dept. of Chemistry

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