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Title: Cooling-induced SUMOylation of EXOSC10 down-regulates ribosome biogenesis.
Authors: Knight, John R. P.
Bastide, Amandine
Peretti, Diego
Roobol, A.
Roobol, J.
Mallucci, Giovanna R.
Smales, C. M.
Willis, Anne E.
First Published: 8-Feb-2016
Publisher: Cold Spring Harbor Laboratory Press for RNA Society
Citation: RNA, 2016, 22 (4), pp. 623-635
Abstract: The RNA exosome is essential for 3' processing of functional RNA species and degradation of aberrant RNAs in eukaryotic cells. Recent reports have defined the substrates of the exosome catalytic domains and solved the multimeric structure of the exosome complex. However, regulation of exosome activity remains poorly characterized, especially in response to physiological stress. Following the observation that cooling of mammalian cells results in a reduction in 40S:60S ribosomal subunit ratio, we uncover regulation of the nuclear exosome as a result of reduced temperature. Using human cells and an in vivo model system allowing whole-body cooling, we observe reduced EXOSC10 (hRrp6, Pm/Scl-100) expression in the cold. In parallel, both models of cooling increase global SUMOylation, leading to the identification of specific conjugation of SUMO1 to EXOSC10, a process that is increased by cooling. Furthermore, we define the major SUMOylation sites in EXOSC10 by mutagenesis and show that overexpression of SUMO1 alone is sufficient to suppress EXOSC10 abundance. Reducing EXOSC10 expression by RNAi in human cells correlates with the 3' preribosomal RNA processing defects seen in the cold as well as reducing the 40S:60S ratio, a previously uncharacterized consequence of EXOSC10 suppression. Together, this work illustrates that EXOSC10 can be modified by SUMOylation and identifies a physiological stress where this regulation is prevalent both in vitro and in vivo.
DOI Link: 10.1261/rna.054411.115
ISSN: 1355-8382
eISSN: 1469-9001
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: © 2016 Knight et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at
Description: Supplemental material is available for this article.
Appears in Collections:Published Articles, Dept. of Molecular and Cell Biology

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