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Title: Reproducibility of fluorescent expression from engineered biological constructs in E. coli
Authors: Beal, J.
Haddock-Angelli, T.
Gershater, M.
De Mora, K.
Lizarazo, M.
Hollenhorst, J.
Rettberg, R.
Demling, P.
Hanke, R.
Osthege, M.
Schechtel, A.
Sudarsan, S.
Zimmermann, A.
Gabryelczyk, B.
Ikonen, M.
Salmela, M.
Acar, M.
Aktas, M. F.
Bestepe, F.
Ceylan, F. S.
Cigdem, S.
Dohan, M.
Elitok, M.
Gunduz, M.
Gunduz, E.
Hatipoglu, O. F.
Kaya, T.
Sayin, O.
Tapan, S.
Tereci, O. F.
Uçar, A.
Yilmaz, M.
Barrick, J.
Gutierrez, A.
Mishler, D.
Monk, J.
Mortensen, K.
Shin, N.
Watkins, E.
Chen, Y.
Jin, Y.
Shi, Y.
Zhang, H. M.
Ono, B.
Paino, I. M. M.
Ribovski, L.
Silva, I.
Zampronio, D. K.
Birkholz, N.
Busche, R. F.
Konzock, O.
Lippold, S.
Ludwig, C.
Philippi, M.
Platz, L.
Sigismund, C.
Weber, S.
Wehrs, M.
Werchau, N.
Wronska, A.
Yen, Z. Z.
Agarwal, Y.
Appleton, E.
Densmore, D.
Esmurria, A.
Lewis, K.
Pacheco, A.
Bruchez, M.
Peters, D.
Telmer, C.
Wang, L.
Canas-Duarte, S.
Giraldo-Perez, D.
Gomez-Garzon, C.
Madrid-Wolff, J.
Marin-Medina, N.
Mazzanti, V.
Rodriguez-Forero, L.
Scher, E.
Dowell, R.
O'Hara, S.
Pogoda, C. S.
Shattuck, K.
Altintas, A.
Bali, A. P.
Bech, R.
Egholm, A.
Hansen, A. S. L.
Jensen, K.
Karlsen, K. B.
Mosbech, C.
Belkhelfa, S.
Berenger, N.
Bodinier, R.
Jacry, C.
First Published: 3-Mar-2016
Publisher: Public Library of Science
Citation: PLoS ONE, 2016, 11(3): e0150182
Abstract: We present results of the first large-scale interlaboratory study carried out in synthetic biology, as part of the 2014 and 2015 International Genetically Engineered Machine (iGEM) competitions. Participants at 88 institutions around the world measured fluorescence from three engineered constitutive constructs in E. coli. Few participants were able to measure absolute fluorescence, so data was analyzed in terms of ratios. Precision was strongly related to fluorescent strength, ranging from 1.54-fold standard deviation for the ratio between strong promoters to 5.75-fold for the ratio between the strongest and weakest promoter, and while host strain did not affect expression ratios, choice of instrument did. This result shows that high quantitative precision and reproducibility of results is possible, while at the same time indicating areas needing improved laboratory practices.
DOI Link: 10.1371/journal.pone.0150182
ISSN: 1932-6203
eISSN: 1932-6203
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: © 2016 Beal et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Appears in Collections:Published Articles, Dept. of Genetics

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