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Title: Surface-enhanced Raman scattering measurement from a lipid bilayer encapsulating a single decahedral nanoparticle mediated by an optical trap
Authors: Wright, A. J.
Richens, J. L.
Bramble, J. P.
Cathcart, N.
Kitaev, V.
O'Shea, P.
Hudson, A. J.
First Published: 2-Sep-2016
Publisher: Royal Society of Chemistry
Citation: Nanoscale, 2016, 8 (36), pp. 16395-16404
Abstract: We present a new technique for the study of model membranes on the length-scale of a single nano-sized liposome. Silver decahedral nanoparticles have been encapsulated by a model unilamellar lipid bilayer creating nano-sized lipid vesicles. The metal core has two roles (i) increasing the polarizability of vesicles, enabling a single vesicle to be isolated and confined in an optical trap, and (ii) enhancing Raman scattering from the bilayer, via the high surface-plasmon field at the sharp vertices of the decahedral particles. Combined this has allowed us to measure a Raman fingerprint from a single vesicle of 50 nm-diameter, containing just ∼104 lipid molecules in a bilayer membrane over a surface area of <0.01 μm2, equivalent to a volume of approximately 1 zepto-litre. Raman scattering is a weak and inefficient process and previous studies have required either a substantially larger bilayer area in order to obtain a detectable signal, or the tagging of lipid molecules with a chromophore to provide an indirect probe of the bilayer. Our approach is fully label-free and bio-compatible and, in the future, it will enable much more localized studies of the heterogeneous structure of lipid bilayers and of membrane-bound components than is currently possible.
DOI Link: 10.1039/c6nr05616d
ISSN: 2040-3364
eISSN: 2040-3372
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Appears in Collections:Published Articles, Dept. of Chemistry

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