Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/38747
Title: A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format.
Authors: Smolinska-Kempisty, Katarzyna
Guerreiro, Antonio
Canfarotta, Francesco
Cáceres, César
Whitcombe, Michael J.
Piletsky, Sergey
First Published: 24-Nov-2016
Publisher: Nature Publishing Group
Citation: Scientific Reports, 2016, 6:37638
Abstract: Here we show that molecularly imprinted polymer nanoparticles, prepared in aqueous media by solid phase synthesis with immobilised L-thyroxine, glucosamine, fumonisin B2 or biotin as template, can demonstrate comparable or better performance to commercially produced antibodies in enzyme-linked competitive assays. Imprinted nanoparticles-based assays showed detection limits in the pM range and polymer-coated microplates are stable to storage at room temperature for at least 1 month. No response to analyte was detected in control experiments with nanoparticles imprinted with an unrelated template (trypsin) but prepared with the same polymer composition. The ease of preparation, high affinity of solid-phase synthesised imprinted nanoparticles and the lack of requirement for cold chain logistics make them an attractive alternative to traditional antibodies for use in immunoassays.
DOI Link: 10.1038/srep37638
ISSN: 2045-2322
eISSN: 2045-2322
Links: http://www.nature.com/articles/srep37638
http://hdl.handle.net/2381/38747
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Appears in Collections:Published Articles, Dept. of Chemistry

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