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Title: Development of an In Vitro Assay for Detection of Drug-Induced Resuscitation-Promoting-Factor-Dependent Mycobacteria.
Authors: Loraine, Jessica
Pu, Feifei
Turapov, Obolbek
Mukamolova, Galina V.
First Published: 8-Aug-2016
Publisher: American Society for Microbiology
Citation: Antimicrobial Agents and Chemotherapy, 2016, 60 (10), pp. 6227-6233 (7)
Abstract: Tuberculosis is a major infectious disease that requires prolonged chemotherapy with a combination of four drugs. Here we present data suggesting that treatment of Mycobacterium tuberculosis, the causative agent of tuberculosis, and Mycobacterium smegmatis, a model organism widely used for the screening of antituberculosis agents, with first-line drugs resulted in the generation of substantial populations that could be recovered only by the addition of a culture supernatant from growing mycobacteria. These bacilli failed to grow in standard media, resulting in significant underestimation of the numbers of viable mycobacteria in treated samples. We generated M. smegmatis strains overexpressing M. tuberculosis resuscitation-promoting factors (Rpfs) and demonstrated their application for the detection of Rpf-dependent mycobacteria generated after drug exposure. Our data offer novel opportunities for validation of the sterilizing activity of antituberculosis agents.
DOI Link: 10.1128/AAC.00518-16
ISSN: 0066-4804
eISSN: 1098-6596
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © the authors, 2016. This is an open-access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Description: Supplemental material for this article may be found at
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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