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Title: Ha-ras restriction fragment length polymorphisms in colorectal cancer.
Authors: Wyllie, F. S.
Wynford-Thomas, V.
Lemoine, N. R.
Williams, G. T.
Williams, E. D.
Wynford-Thomas, D.
First Published: Feb-1988
Publisher: Cancer Research UK, Nature Publishing Group
Citation: British Journal of Cancer, 1988, 57 (2), pp. 135-138
Abstract: The possibility of an association between restriction fragment length polymorphisms (RFLPs) at the Ha-ras gene locus and susceptibility to develop colorectal cancer has been investigated. Leucocyte DNA from 46 carcinoma patients and 49 controls was analysed by Southern blotting to determine the size distribution of restriction fragments containing the variable tandem repeat 3' to the Ha-ras gene. Four predominant allelic fragments were found in both groups (in AvaII digests having sizes of 1.55, 2.0, 2.65 and 3.15 kilobases [kb]), together with a variety of 'rare' alleles (with individual frequencies less than 5%). The overall prevalence of rare alleles was not significantly different between cancer and control groups. The distribution of the common alleles, however, differed significantly. The combined frequency of the two larger alleles (a3 and a4) was approximately twice as high in the cancer group (34%) as in controls (18%) (P less than 0.025), which was reflected in a highly significant increase in the proportion of individuals carrying an a3 or a4 allele.
DOI Link: 10.1038/bjc.1988.28
ISSN: 0007-0920
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Creative Commons “Attribution Non-Commercial No Derivatives” licence CC BY-NC-ND, further details of which can be found via the following link: Archived with reference to SHERPA/RoMEO and publisher website.
Appears in Collections:Published Articles, College of Medicine, Biological Sciences and Psychology

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