Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/38935
Title: Evaluation of Antigens for Development of a Serological Test for Human African Trypanosomiasis
Authors: Biéler, S.
Waltenberger, H.
Barrett, M. P.
McCulloch, R.
Mottram, J. C.
Carrington, M.
Schwaeble, Wilhelm
McKerrow, J.
Phillips, M. A.
Michels, P. A.
Büscher, P.
Sanchez, J-C.
Bishop, R.
Robinson, D. R.
Bangs, J.
Ferguson, M.
Nerima, B.
Albertini, A.
Michel, G.
Radwandska, M.
Mathu Ndung’u, J.
First Published: 9-Dec-2016
Publisher: Public Library of Science
Citation: PLoS One, 2016 11(12): e0168074.
Abstract: Background Control and elimination of human African trypanosomiasis (HAT) can be accelerated through the use of diagnostic tests that are more accurate and easier to deploy. The goal of this work was to evaluate the immuno-reactivity of antigens and identify candidates to be considered for development of a simple serological test for the detection of Trypanosoma brucei gambiense or T. b. rhodesiense infections, ideally both. Methodology/Principal Findings The reactivity of 35 antigens was independently evaluated by slot blot and ELISA against sera from both T. b. gambiense and T. b. rhodesiense infected patients and controls. The antigens that were most reactive by both tests to T. b. gambiense sera were the membrane proteins VSG LiTat 1.3, VSG LiTat 1.5 and ISG64. Reactivity to T. b. rhodesiense sera was highest with VSG LiTat 1.3, VSG LiTat 1.5 and SRA, although much lower than with T. b. gambiense samples. The reactivity of all possible combinations of antigens was also calculated. When the slot blot results of 2 antigens were paired, a VSG LiTat 1.3- ISG75 combination performed best on T. b. gambiense sera, while a VSG LiTat 1.3-VSG LiTat 1.5 combination was the most reactive using ELISA. A combination of SRA and either VSG LiTat 1.3 or VSG LiTat 1.5 had the highest reactivity on T. b. rhodesiense sera according to slot blot, while in ELISA, pairing SRA with either GM6 or VSG LiTat 1.3 yielded the best results. Conclusions This study identified antigens that were highly reactive to T. b. gambiense sera, which could be considered for developing a serological test for gambiense HAT, either individually or in combination. Antigens with potential for inclusion in a test for T. b. rhodesiense HAT were also identified, but because their reactivity was comparatively lower, a search for additional antigens would be required before developing a test for this form of the disease.
DOI Link: 10.1371/journal.pone.0168074
ISSN: 1932-6203
Links: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0168074
http://hdl.handle.net/2381/38935
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: © 2016 Biéler et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

Files in This Item:
File Description SizeFormat 
journal.pone.0168074.pdfPublished (publisher PDF)4.57 MBAdobe PDFView/Open


Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.