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Title: Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection.
Authors: Lemoine, N. R.
Mayall, E. S.
Jones, T.
Sheer, D.
McDermid, S.
Kendall-Taylor, P.
Wynford-Thomas, D.
First Published: Dec-1989
Publisher: Cancer Research UK, Nature Publishing Group
Citation: British Journal of Cancer (1989) 60, 897–903.
Abstract: Human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SVori-) to produce several immortal cell lines. Two of the 10 cell lines analysed expressed specific features of thyroid epithelial function (iodide-trapping and thyroglobulin production). These two lines were characterised in detail and found to be growth factor-independent, capable of anchorage-independent growth at low frequency but non-tumorigenic in nude mice. These differentiated, These differentiated, partially transformed cell lines were shown to be suitable for gene transfer at high frequency using simple coprecipitation techniques.
DOI Link: 10.1038/bjc.1989.387
ISSN: 0007-0920
eISSN: 1532-1827
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Creative Commons “Attribution Non-Commercial No Derivatives” licence CC BY-NC-ND, further details of which can be found via the following link: Archived with reference to SHERPA/RoMEO and publisher website.
Appears in Collections:Published Articles, College of Medicine, Biological Sciences and Psychology

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