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|Title:||Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection.|
|Authors:||Lemoine, N. R.|
Mayall, E. S.
|Publisher:||Cancer Research UK, Nature Publishing Group|
|Citation:||British Journal of Cancer (1989) 60, 897–903.|
|Abstract:||Human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SVori-) to produce several immortal cell lines. Two of the 10 cell lines analysed expressed specific features of thyroid epithelial function (iodide-trapping and thyroglobulin production). These two lines were characterised in detail and found to be growth factor-independent, capable of anchorage-independent growth at low frequency but non-tumorigenic in nude mice. These differentiated, These differentiated, partially transformed cell lines were shown to be suitable for gene transfer at high frequency using simple coprecipitation techniques.|
|Rights:||Creative Commons “Attribution Non-Commercial No Derivatives” licence CC BY-NC-ND, further details of which can be found via the following link: http://creativecommons.org/licenses/by-nc-nd/4.0/ Archived with reference to SHERPA/RoMEO and publisher website.|
|Appears in Collections:||Published Articles, College of Medicine, Biological Sciences and Psychology|
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