Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/38948
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dc.contributor.authorLemoine, N. R.-
dc.contributor.authorMayall, E. S.-
dc.contributor.authorJones, T.-
dc.contributor.authorSheer, D.-
dc.contributor.authorMcDermid, S.-
dc.contributor.authorKendall-Taylor, P.-
dc.contributor.authorWynford-Thomas, D.-
dc.date.accessioned2016-12-14T16:49:46Z-
dc.date.available2016-12-14T16:49:46Z-
dc.date.issued1989-12-
dc.identifier.citationBritish Journal of Cancer (1989) 60, 897–903.en
dc.identifier.issn0007-0920-
dc.identifier.urihttp://www.nature.com/bjc/journal/v60/n6/abs/bjc1989387a.htmlen
dc.identifier.urihttp://hdl.handle.net/2381/38948-
dc.description.abstractHuman primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SVori-) to produce several immortal cell lines. Two of the 10 cell lines analysed expressed specific features of thyroid epithelial function (iodide-trapping and thyroglobulin production). These two lines were characterised in detail and found to be growth factor-independent, capable of anchorage-independent growth at low frequency but non-tumorigenic in nude mice. These differentiated, These differentiated, partially transformed cell lines were shown to be suitable for gene transfer at high frequency using simple coprecipitation techniques.en
dc.description.sponsorshipThis work was supported by a grant from the Welsh Scheme for the Development of Health Service Resources.en
dc.language.isoenen
dc.publisherCancer Research UK, Nature Publishing Groupen
dc.relation.urihttp://www.ncbi.nlm.nih.gov/pubmed/2557880-
dc.rightsCreative Commons “Attribution Non-Commercial No Derivatives” licence CC BY-NC-ND, further details of which can be found via the following link: http://creativecommons.org/licenses/by-nc-nd/4.0/ Archived with reference to SHERPA/RoMEO and publisher website.en
dc.subjectAdulten
dc.subjectAnimalsen
dc.subjectCell Lineen
dc.subjectDNA, Viralen
dc.subjectEpitheliumen
dc.subjectFemaleen
dc.subjectGenetic Markersen
dc.subjectHumansen
dc.subjectKaryotypingen
dc.subjectMiceen
dc.subjectMice, Nudeen
dc.subjectSimian virus 40en
dc.subjectSodium Iodideen
dc.subjectThyroglobulinen
dc.subjectThyroid Glanden
dc.subjectTransfectionen
dc.titleCharacterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection.en
dc.typeJournal Articleen
dc.identifier.doi10.1038/bjc.1989.387-
dc.identifier.eissn1532-1827-
dc.description.statusPeer-revieweden
dc.description.versionPublisher Versionen
dc.type.subtypeJournal Article;Research Support, Non-U.S. Gov't-
pubs.organisational-group/Organisationen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGYen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicineen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/Themesen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/Themes/No themeen
Appears in Collections:Published Articles, College of Medicine, Biological Sciences and Psychology

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