Please use this identifier to cite or link to this item:
|Title:||Role of Stat3 in Glucocorticoid-induced Expression of the human IL-10 Gene|
Staples, Karl J.
Williams, Lynn M.
Hofer, T. P. J.
|Citation:||Molecular Immunology, 2008, 45 (11), pp. 3230-3237.|
|Abstract:||In the present report we have determined the molecular mechanisms, which govern the expression of the human IL-10 gene when induced by the glucocorticoid Methyl-Prednisolone (MP). Treatment cells with MP at 10-6 M will readily induce IL-10 in CD19+ primary B cells and in a human B cell line. Analysis of the IL-10 promoter showed a robust 18-fold induction and demonstrated that a potential GRE motif was not required, while mutation of the - 120 STAT-motif strongly reduced MP induced transactivation. A strong induction was also seen with a trimeric STAT-motif and over-expression of dominantnegative STAT3 could block MP induction of IL-10 mRNA. Finally, MP treatment induced binding of STAT3 to the promoter as shown by gelshift, supershift and by chromatin-immuno-precipitation. These data show that glucocorticoid-induced expression of the IL-10 gene is mediated by the transcription factor STAT3.|
|Rights:||This is the author's final draft of the paper published as Molecular Immunology, 2008, 45 (11) pp. 3230-3237. The final published version is available from www.sciencedirect.com. Doi:10.1016/j.molimm.2008.02.020|
|Appears in Collections:||Published Articles, Dept. of Infection, Immunity and Inflammation|
Files in This Item:
|MIMM-D-07-00686 revised Unterberger GC IL-10MIF.pdf||406.1 kB||Adobe PDF||View/Open|
Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.