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Title: Amplification of human platelet activation by surface pannexin-1 channels
Authors: Taylor, K. A.
Wright, J. R.
Vial, C.
Evans, R. J.
Mahaut-Smith, M. P.
First Published: 10-Jun-2014
Publisher: Wiley for International Society on Thrombosis and Haemostasis
Citation: Journal of Thrombosis and Haemostasis, 2014, 12 (6), pp. 987-998 (12)
Abstract: Background: Pannexin-1 (Panx1) forms an anionselective channel with a permeability up to ~1 kDa and represents a non-lytic, non-vesicular ATP release pathway in erythrocytes, leukocytes and neurons. Related connexin gap junction proteins have been reported in platelets; however, the expression and function of the pannexins remain unknown. Objective: To determine the expression and function of pannexins in human platelets, using molecular, cellular and functional techniques. Methods: Panx1 expression in human platelets was determined using qPCR and antibody-based techniques. Contributions of Panx1 to agonist-evoked efflux of cytoplasmic calcein, Ca2+ influx, ATP release and aggregation were assessed in washed platelets under conditions where the P2X1 receptor response was preserved (0.32 U mL 1 apyrase). Thrombus formation in whole blood was assessed in vitro using a shear chamber assay. Two structurally unrelated and widely used Panx1 inhibitors, probenecid and carbenoxolone, were used throughout this study, at concentrations that do not affect connexin channels. Results: PANX1, but not PANX2 or PANX3, mRNA was detected in human platelets. Furthermore, Panx1 protein is glycosylated and present on the plasma membrane of platelets, and displays weak physical association with P2X1 receptors. Panx1 inhibition blocked thrombinevoked efflux of calcein, and reduced Ca2+ influx, ATP release, platelet aggregation and thrombus formation under arterial shear rates in vitro. The Panx1-dependent contribution was not additive to that of P2X1 receptors. Conclusions: Panx1 is expressed on human platelets and amplifies Ca2+ influx, ATP release and aggregation
DOI Link: 10.1111/jth.12566
ISSN: 1538-7933
eISSN: 1538-7836
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: © 2014 The Authors. Journal of Thrombosis and Haemostasis published by Wiley Periodicals, Inc. on behalf of International Society on Thrombosis and Haemostasis This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Appears in Collections:Published Articles, Dept. of Molecular and Cell Biology

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