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|Title:||Pigment composition and adaptation in free-living and symbiotic strains of Acaryochloris marina|
Clokie, Samuel J. H.
Mann, Nicholas H.
Scanlan, David J.
Whitworth, Anna L.
Clokie, Martha R. J.
|Citation:||FEMS Microbiology Ecology, 2007, 61(1), pp.65-73|
|Abstract:||Acaryochloris marina strains have been isolated from several varied locations and habitats worldwide, thus demonstrating a diverse and dynamic ecology. In this study, the whole cell photophysiologies of strain MBIC11017, originally isolated from a colonial ascidian, and the free-living epilithic strain CCMEE5410 are analysed by absorbance and fluorescence spectroscopy, laser scanning confocal microscopy, SDS-PAGE and subsequent protein analysis. We demonstrate pigment adaptation in MBIC11017 and CCMEE5410 under different light regimes. We show that strain MBIC11017 loses its phycobilins relative to its chlorophyll d content when grown at light intensities of 40 μE m-2 s-1 without shaking and 100 μE m-2 s-1 with shaking. Our results indicate the greater the light intensity both strains MBIC11017 and CCMEE5410 are grown at, the greater the decrease in their chlorophyll d content. We also conclude that phycobiliproteins are absent in the free-living strain CCMEE5410.|
|Rights:||This is the authors' final draft of the paper published as FEMS Microbiology Ecology, 207, 61(1), pp.65-73. The final published version is available from http://www3.interscience.wiley.com/journal/118504120/abstract|
|Appears in Collections:||Published Articles, Dept. of Infection, Immunity and Inflammation|
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