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Title: Rapamycin regulates biochemical metabolites.
Authors: Tucci, Paola
Porta, Giovanni
Agostini, Massimiliano
Antonov, Alexey
Garabadgiu, Alexander Vasilievich
Melino, Gerry
Willis, Anne E.
First Published: 28-Jun-2013
Publisher: Taylor & Francis
Citation: Cell Cycle, 2013, 12 (15), pp. 2454-2467
Abstract: The mammalian target of rapamycin (mTOR) kinase is a master regulator of protein synthesis that couples nutrient sensing to cell growth, and deregulation of this pathway is associated with tumorigenesis. p53, and its less investigated family member p73, have been shown to interact closely with mTOR pathways through the transcriptional regulation of different target genes. To investigate the metabolic changes that occur upon inhibition of the mTOR pathway and the role of p73 in this response primary mouse embryonic fibroblast from control and TAp73(-/-) were treated with the macrocyclic lactone rapamycin. Extensive gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS/MS) analysis were used to obtain a rapamycin-dependent global metabolome profile from control or TAp73(-/-) cells. In total 289 metabolites involved in selective pathways were identified; 39 biochemical metabolites were found to be significantly altered, many of which are known to be associated with the cellular stress response.
DOI Link: 10.4161/cc.25450
ISSN: 1538-4101
eISSN: 1551-4005
Version: Post-print
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2013, Taylor & Francis. Deposited with reference to the publisher’s open access archiving policy.
Description: Supplemental materials may be found here:
Appears in Collections:Published Articles, Dept. of Molecular and Cell Biology

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