Please use this identifier to cite or link to this item:
|Title:||Rapamycin regulates biochemical metabolites.|
Garabadgiu, Alexander Vasilievich
Willis, Anne E.
|Publisher:||Taylor & Francis|
|Citation:||Cell Cycle, 2013, 12 (15), pp. 2454-2467|
|Abstract:||The mammalian target of rapamycin (mTOR) kinase is a master regulator of protein synthesis that couples nutrient sensing to cell growth, and deregulation of this pathway is associated with tumorigenesis. p53, and its less investigated family member p73, have been shown to interact closely with mTOR pathways through the transcriptional regulation of different target genes. To investigate the metabolic changes that occur upon inhibition of the mTOR pathway and the role of p73 in this response primary mouse embryonic fibroblast from control and TAp73(-/-) were treated with the macrocyclic lactone rapamycin. Extensive gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS/MS) analysis were used to obtain a rapamycin-dependent global metabolome profile from control or TAp73(-/-) cells. In total 289 metabolites involved in selective pathways were identified; 39 biochemical metabolites were found to be significantly altered, many of which are known to be associated with the cellular stress response.|
|Rights:||Copyright © 2013, Taylor & Francis. Deposited with reference to the publisher’s open access archiving policy.|
|Description:||Supplemental materials may be found here: www.landesbioscience.com/journals/cc/article/25450|
|Appears in Collections:||Published Articles, Dept. of Molecular and Cell Biology|
Files in This Item:
|Rapamycin regulates biochemical metabolites..pdf||Published (publisher PDF)||3.11 MB||Adobe PDF||View/Open|
Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.